Cargando…

The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions

Peroxiredoxins (Prxs) are thiol-specific antioxidant proteins that exhibit peroxidase and peroxynitrite reductase activities involved in the reduction of reactive oxygen species. The peroxiredoxin Prx4 from the large yellow croaker Pseudosciaena crocea is a typical 2-Cys Prx with an N-terminal signa...

Descripción completa

Detalles Bibliográficos
Autores principales: Mu, Yinnan, Lian, Fu-Ming, Teng, Yan-Bin, Ao, Jingqun, Jiang, Yong-Liang, He, Yong-Xing, Chen, Yuxing, Zhou, Cong-Zhao, Chen, Xinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581551/
https://www.ncbi.nlm.nih.gov/pubmed/23451146
http://dx.doi.org/10.1371/journal.pone.0057061
_version_ 1782260435625443328
author Mu, Yinnan
Lian, Fu-Ming
Teng, Yan-Bin
Ao, Jingqun
Jiang, Yong-Liang
He, Yong-Xing
Chen, Yuxing
Zhou, Cong-Zhao
Chen, Xinhua
author_facet Mu, Yinnan
Lian, Fu-Ming
Teng, Yan-Bin
Ao, Jingqun
Jiang, Yong-Liang
He, Yong-Xing
Chen, Yuxing
Zhou, Cong-Zhao
Chen, Xinhua
author_sort Mu, Yinnan
collection PubMed
description Peroxiredoxins (Prxs) are thiol-specific antioxidant proteins that exhibit peroxidase and peroxynitrite reductase activities involved in the reduction of reactive oxygen species. The peroxiredoxin Prx4 from the large yellow croaker Pseudosciaena crocea is a typical 2-Cys Prx with an N-terminal signal peptide. We solved the crystal structure of Prx4 at 1.90 Å and revealed an N-terminal antiparallel β-sheet that contributes to the dimer interface. Deletion of this β-sheet decreased the in vitro peroxidase activity to about 50% of the wild-type. In vivo assays further demonstrated that removal of this β-sheet led to some impairment in the ability of Prx4 to negatively regulate nuclear factor-κB (NF-κB) activity and to perform its role in anti-bacterial immunity. These results provide new insights into the structure and function relationship of a peroxiredoxin from bony fish.
format Online
Article
Text
id pubmed-3581551
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35815512013-02-28 The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions Mu, Yinnan Lian, Fu-Ming Teng, Yan-Bin Ao, Jingqun Jiang, Yong-Liang He, Yong-Xing Chen, Yuxing Zhou, Cong-Zhao Chen, Xinhua PLoS One Research Article Peroxiredoxins (Prxs) are thiol-specific antioxidant proteins that exhibit peroxidase and peroxynitrite reductase activities involved in the reduction of reactive oxygen species. The peroxiredoxin Prx4 from the large yellow croaker Pseudosciaena crocea is a typical 2-Cys Prx with an N-terminal signal peptide. We solved the crystal structure of Prx4 at 1.90 Å and revealed an N-terminal antiparallel β-sheet that contributes to the dimer interface. Deletion of this β-sheet decreased the in vitro peroxidase activity to about 50% of the wild-type. In vivo assays further demonstrated that removal of this β-sheet led to some impairment in the ability of Prx4 to negatively regulate nuclear factor-κB (NF-κB) activity and to perform its role in anti-bacterial immunity. These results provide new insights into the structure and function relationship of a peroxiredoxin from bony fish. Public Library of Science 2013-02-25 /pmc/articles/PMC3581551/ /pubmed/23451146 http://dx.doi.org/10.1371/journal.pone.0057061 Text en © 2013 Mu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mu, Yinnan
Lian, Fu-Ming
Teng, Yan-Bin
Ao, Jingqun
Jiang, Yong-Liang
He, Yong-Xing
Chen, Yuxing
Zhou, Cong-Zhao
Chen, Xinhua
The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions
title The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions
title_full The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions
title_fullStr The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions
title_full_unstemmed The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions
title_short The N-Terminal β-Sheet of Peroxiredoxin 4 in the Large Yellow Croaker Pseudosciaena crocea Is Involved in Its Biological Functions
title_sort n-terminal β-sheet of peroxiredoxin 4 in the large yellow croaker pseudosciaena crocea is involved in its biological functions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581551/
https://www.ncbi.nlm.nih.gov/pubmed/23451146
http://dx.doi.org/10.1371/journal.pone.0057061
work_keys_str_mv AT muyinnan thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT lianfuming thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT tengyanbin thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT aojingqun thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT jiangyongliang thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT heyongxing thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT chenyuxing thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT zhoucongzhao thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT chenxinhua thenterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT muyinnan nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT lianfuming nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT tengyanbin nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT aojingqun nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT jiangyongliang nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT heyongxing nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT chenyuxing nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT zhoucongzhao nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions
AT chenxinhua nterminalbsheetofperoxiredoxin4inthelargeyellowcroakerpseudosciaenacroceaisinvolvedinitsbiologicalfunctions