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A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES

Translation initiation from the human immunodeficiency virus type-1 (HIV-1) mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS)-1, a cis-acting regulatory element present within the gag...

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Autores principales: Valiente-Echeverría, Fernando, Vallejos, Maricarmen, Monette, Anne, Pino, Karla, Letelier, Alejandro, Huidobro-Toro, J. Pablo, Mouland, Andrew J., López-Lastra, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581557/
https://www.ncbi.nlm.nih.gov/pubmed/23451120
http://dx.doi.org/10.1371/journal.pone.0056962
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author Valiente-Echeverría, Fernando
Vallejos, Maricarmen
Monette, Anne
Pino, Karla
Letelier, Alejandro
Huidobro-Toro, J. Pablo
Mouland, Andrew J.
López-Lastra, Marcelo
author_facet Valiente-Echeverría, Fernando
Vallejos, Maricarmen
Monette, Anne
Pino, Karla
Letelier, Alejandro
Huidobro-Toro, J. Pablo
Mouland, Andrew J.
López-Lastra, Marcelo
author_sort Valiente-Echeverría, Fernando
collection PubMed
description Translation initiation from the human immunodeficiency virus type-1 (HIV-1) mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS)-1, a cis-acting regulatory element present within the gag open reading frame (ORF). In this study we evaluated the impact of the INS-1 on HIV-1 IRES-mediated translation initiation. Using heterologous bicistronic mRNAs, we show that the INS-1 negatively impact on HIV-1 IRES-driven translation in in vitro and in cell-based experiments. Additionally, our results show that the inhibitory effect of the INS-1 is not general to all IRESes since it does not hinder translation driven by the HCV IRES. The inhibition by the INS-1 was partially rescued in cells by the overexpression of the viral Rev protein or hnRNPA1.
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spelling pubmed-35815572013-02-28 A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES Valiente-Echeverría, Fernando Vallejos, Maricarmen Monette, Anne Pino, Karla Letelier, Alejandro Huidobro-Toro, J. Pablo Mouland, Andrew J. López-Lastra, Marcelo PLoS One Research Article Translation initiation from the human immunodeficiency virus type-1 (HIV-1) mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS)-1, a cis-acting regulatory element present within the gag open reading frame (ORF). In this study we evaluated the impact of the INS-1 on HIV-1 IRES-mediated translation initiation. Using heterologous bicistronic mRNAs, we show that the INS-1 negatively impact on HIV-1 IRES-driven translation in in vitro and in cell-based experiments. Additionally, our results show that the inhibitory effect of the INS-1 is not general to all IRESes since it does not hinder translation driven by the HCV IRES. The inhibition by the INS-1 was partially rescued in cells by the overexpression of the viral Rev protein or hnRNPA1. Public Library of Science 2013-02-25 /pmc/articles/PMC3581557/ /pubmed/23451120 http://dx.doi.org/10.1371/journal.pone.0056962 Text en © 2013 Valiente-Echeverría et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Valiente-Echeverría, Fernando
Vallejos, Maricarmen
Monette, Anne
Pino, Karla
Letelier, Alejandro
Huidobro-Toro, J. Pablo
Mouland, Andrew J.
López-Lastra, Marcelo
A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES
title A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES
title_full A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES
title_fullStr A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES
title_full_unstemmed A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES
title_short A cis-Acting Element Present within the gag Open Reading Frame Negatively Impacts on the Activity of the HIV-1 IRES
title_sort cis-acting element present within the gag open reading frame negatively impacts on the activity of the hiv-1 ires
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581557/
https://www.ncbi.nlm.nih.gov/pubmed/23451120
http://dx.doi.org/10.1371/journal.pone.0056962
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