Cargando…
Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries
Compartments are units of cell lineage that subdivide territories with different developmental potential. In Drosophila, the wing and haltere discs are subdivided into anterior and posterior (A/P) compartments, which require the activity of Hedgehog, and into dorsal and ventral (D/V) compartments, n...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581558/ https://www.ncbi.nlm.nih.gov/pubmed/23451173 http://dx.doi.org/10.1371/journal.pone.0057159 |
_version_ | 1782260437300019200 |
---|---|
author | Curt, Jesús R. de Navas, Luis F. Sánchez-Herrero, Ernesto |
author_facet | Curt, Jesús R. de Navas, Luis F. Sánchez-Herrero, Ernesto |
author_sort | Curt, Jesús R. |
collection | PubMed |
description | Compartments are units of cell lineage that subdivide territories with different developmental potential. In Drosophila, the wing and haltere discs are subdivided into anterior and posterior (A/P) compartments, which require the activity of Hedgehog, and into dorsal and ventral (D/V) compartments, needing Notch signaling. There is enrichment in actomyosin proteins at the compartment boundaries, suggesting a role for these proteins in their maintenance. Compartments also develop in the mouse hindbrain rhombomeres, which are characterized by the expression of different Hox genes, a group of genes specifying different structures along their main axis of bilaterians. We show here that the Drosophila Hox gene Ultrabithorax can maintain the A/P and D/V compartment boundaries when Hedgehog or Notch signaling is compromised, and that the interaction of cells with and without Ultrabithorax expression induces high levels of non-muscle myosin II. In the absence of Ultrabithorax there is occasional mixing of cells from different segments. We also show a similar role in cell segregation for the Abdominal-B Hox gene. Our results suggest that the juxtaposition of cells with different Hox gene expression leads to their sorting out, probably through the accumulation of non-muscle myosin II at the boundary of the different cell territories. The increase in myosin expression seems to be a general mechanism used by Hox genes or signaling pathways to maintain the segregation of different groups of cells. |
format | Online Article Text |
id | pubmed-3581558 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35815582013-02-28 Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries Curt, Jesús R. de Navas, Luis F. Sánchez-Herrero, Ernesto PLoS One Research Article Compartments are units of cell lineage that subdivide territories with different developmental potential. In Drosophila, the wing and haltere discs are subdivided into anterior and posterior (A/P) compartments, which require the activity of Hedgehog, and into dorsal and ventral (D/V) compartments, needing Notch signaling. There is enrichment in actomyosin proteins at the compartment boundaries, suggesting a role for these proteins in their maintenance. Compartments also develop in the mouse hindbrain rhombomeres, which are characterized by the expression of different Hox genes, a group of genes specifying different structures along their main axis of bilaterians. We show here that the Drosophila Hox gene Ultrabithorax can maintain the A/P and D/V compartment boundaries when Hedgehog or Notch signaling is compromised, and that the interaction of cells with and without Ultrabithorax expression induces high levels of non-muscle myosin II. In the absence of Ultrabithorax there is occasional mixing of cells from different segments. We also show a similar role in cell segregation for the Abdominal-B Hox gene. Our results suggest that the juxtaposition of cells with different Hox gene expression leads to their sorting out, probably through the accumulation of non-muscle myosin II at the boundary of the different cell territories. The increase in myosin expression seems to be a general mechanism used by Hox genes or signaling pathways to maintain the segregation of different groups of cells. Public Library of Science 2013-02-25 /pmc/articles/PMC3581558/ /pubmed/23451173 http://dx.doi.org/10.1371/journal.pone.0057159 Text en © 2013 Curt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Curt, Jesús R. de Navas, Luis F. Sánchez-Herrero, Ernesto Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries |
title | Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries |
title_full | Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries |
title_fullStr | Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries |
title_full_unstemmed | Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries |
title_short | Differential Activity of Drosophila Hox Genes Induces Myosin Expression and Can Maintain Compartment Boundaries |
title_sort | differential activity of drosophila hox genes induces myosin expression and can maintain compartment boundaries |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581558/ https://www.ncbi.nlm.nih.gov/pubmed/23451173 http://dx.doi.org/10.1371/journal.pone.0057159 |
work_keys_str_mv | AT curtjesusr differentialactivityofdrosophilahoxgenesinducesmyosinexpressionandcanmaintaincompartmentboundaries AT denavasluisf differentialactivityofdrosophilahoxgenesinducesmyosinexpressionandcanmaintaincompartmentboundaries AT sanchezherreroernesto differentialactivityofdrosophilahoxgenesinducesmyosinexpressionandcanmaintaincompartmentboundaries |