Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells

BACKGROUND: Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression...

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Detalles Bibliográficos
Autores principales: Huang, Bo, Zhou, Hongli, Wang, Xiaodong, Liu, Zhiliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Primary Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3582488/
https://www.ncbi.nlm.nih.gov/pubmed/23379909
http://dx.doi.org/10.1186/1475-2867-13-8
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author Huang, Bo
Zhou, Hongli
Wang, Xiaodong
Liu, Zhiliang
author_facet Huang, Bo
Zhou, Hongli
Wang, Xiaodong
Liu, Zhiliang
author_sort Huang, Bo
collection PubMed
description BACKGROUND: Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression of SATB1 in SCLC. METHODS: The level of SATB1 was analyzed in SCLC tissues, metastatic lymphoid nodes and adjacent normal lung tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA was constructed and transfected into human SCLC cell line NCI-H446 to evaluate the effects of SATB1-siRNA on cell proliferation, invasion and apoptosis. RESULT: SATB1 protein was overexpressed in SCLC tissues and metastasis lymphoid nodes compared with adjacent normal lung tissues. Compared with control group, SATB1-siRNA inhibits the proliferation and invasion of SCLC cells and induces SCLC cells apoptosis statistically (P<0.05) in vitro. CONCLUSION: Our results suggest that SATB1 plays an important role in the metastasis of human SCLC cell.
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spelling pubmed-35824882013-02-27 Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells Huang, Bo Zhou, Hongli Wang, Xiaodong Liu, Zhiliang Cancer Cell Int Primary Research BACKGROUND: Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression of SATB1 in SCLC. METHODS: The level of SATB1 was analyzed in SCLC tissues, metastatic lymphoid nodes and adjacent normal lung tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA was constructed and transfected into human SCLC cell line NCI-H446 to evaluate the effects of SATB1-siRNA on cell proliferation, invasion and apoptosis. RESULT: SATB1 protein was overexpressed in SCLC tissues and metastasis lymphoid nodes compared with adjacent normal lung tissues. Compared with control group, SATB1-siRNA inhibits the proliferation and invasion of SCLC cells and induces SCLC cells apoptosis statistically (P<0.05) in vitro. CONCLUSION: Our results suggest that SATB1 plays an important role in the metastasis of human SCLC cell. BioMed Central 2013-02-05 /pmc/articles/PMC3582488/ /pubmed/23379909 http://dx.doi.org/10.1186/1475-2867-13-8 Text en Copyright ©2013 Huang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primary Research
Huang, Bo
Zhou, Hongli
Wang, Xiaodong
Liu, Zhiliang
Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
title Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
title_full Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
title_fullStr Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
title_full_unstemmed Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
title_short Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
title_sort silencing satb1 with sirna inhibits the proliferation and invasion of small cell lung cancer cells
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3582488/
https://www.ncbi.nlm.nih.gov/pubmed/23379909
http://dx.doi.org/10.1186/1475-2867-13-8
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AT wangxiaodong silencingsatb1withsirnainhibitstheproliferationandinvasionofsmallcelllungcancercells
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