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A comparison of E15.5 fetus and newborn rat serum proteomes
BACKGROUND: Serum proteins carry out several functions in the circulation, including transfer, immunological functions, messenger functions, coagulation, and regulation of homeostasis. To investigate changes in serum proteins that occur during development, the serum proteomes of embryonic 15.5 (E15....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583134/ https://www.ncbi.nlm.nih.gov/pubmed/23134622 http://dx.doi.org/10.1186/1477-5956-10-64 |
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author | Wei, Lilong Jia, Lulu Zhu, Lisi Ma, Sucan Zhang, Dan Shao, Chen Sun, Wei Gao, Youhe |
author_facet | Wei, Lilong Jia, Lulu Zhu, Lisi Ma, Sucan Zhang, Dan Shao, Chen Sun, Wei Gao, Youhe |
author_sort | Wei, Lilong |
collection | PubMed |
description | BACKGROUND: Serum proteins carry out several functions in the circulation, including transfer, immunological functions, messenger functions, coagulation, and regulation of homeostasis. To investigate changes in serum proteins that occur during development, the serum proteomes of embryonic 15.5 (E15.5) fetuses and newborn rats were compared using LC-MS/MS. RESULTS: A total of 958 proteins were identified in the serum of rats at both developmental stages. The serum proteome pattern of newborn rats was compared to E15.5 fetuses by relative quantitation. The expression patterns of hemoglobin subunits were different at the two stages, with most of the subunits having decreased expression in newborn rats compared to E15.5 fetuses. In addition, 8 of 12 apolipoproteins were significantly decreased and 10 of 11 identified complement molecules were increased, with 4 exhibiting a significant increase. Moreover, 11 of 14 of the significantly increased enzyme regulators were inhibitors. The serum proteome patterns of different littermates from both developmental stages were also compared. We found that the levels of many highly abundant serum proteins varied between littermates, and the variations were larger than the variations of the technical control. CONCLUSIONS: The serum proteomes of newborn rats and E15.5 fetuses were compared. The expression patterns of hemoglobin subunits were different at the two developmental stages, with most of the subunits having decreased expression. The majority of apolipoproteins had significantly decreased expression, while almost all identified complement proteins had increased expression. The levels of several highly abundant serum proteins also varied among littermates at these two developmental stages. This is the first study using LC-MS/MS to investigate serum proteome development. |
format | Online Article Text |
id | pubmed-3583134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35831342013-02-28 A comparison of E15.5 fetus and newborn rat serum proteomes Wei, Lilong Jia, Lulu Zhu, Lisi Ma, Sucan Zhang, Dan Shao, Chen Sun, Wei Gao, Youhe Proteome Sci Research BACKGROUND: Serum proteins carry out several functions in the circulation, including transfer, immunological functions, messenger functions, coagulation, and regulation of homeostasis. To investigate changes in serum proteins that occur during development, the serum proteomes of embryonic 15.5 (E15.5) fetuses and newborn rats were compared using LC-MS/MS. RESULTS: A total of 958 proteins were identified in the serum of rats at both developmental stages. The serum proteome pattern of newborn rats was compared to E15.5 fetuses by relative quantitation. The expression patterns of hemoglobin subunits were different at the two stages, with most of the subunits having decreased expression in newborn rats compared to E15.5 fetuses. In addition, 8 of 12 apolipoproteins were significantly decreased and 10 of 11 identified complement molecules were increased, with 4 exhibiting a significant increase. Moreover, 11 of 14 of the significantly increased enzyme regulators were inhibitors. The serum proteome patterns of different littermates from both developmental stages were also compared. We found that the levels of many highly abundant serum proteins varied between littermates, and the variations were larger than the variations of the technical control. CONCLUSIONS: The serum proteomes of newborn rats and E15.5 fetuses were compared. The expression patterns of hemoglobin subunits were different at the two developmental stages, with most of the subunits having decreased expression. The majority of apolipoproteins had significantly decreased expression, while almost all identified complement proteins had increased expression. The levels of several highly abundant serum proteins also varied among littermates at these two developmental stages. This is the first study using LC-MS/MS to investigate serum proteome development. BioMed Central 2012-11-07 /pmc/articles/PMC3583134/ /pubmed/23134622 http://dx.doi.org/10.1186/1477-5956-10-64 Text en Copyright © 2012 Wei et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Wei, Lilong Jia, Lulu Zhu, Lisi Ma, Sucan Zhang, Dan Shao, Chen Sun, Wei Gao, Youhe A comparison of E15.5 fetus and newborn rat serum proteomes |
title | A comparison of E15.5 fetus and newborn rat serum proteomes |
title_full | A comparison of E15.5 fetus and newborn rat serum proteomes |
title_fullStr | A comparison of E15.5 fetus and newborn rat serum proteomes |
title_full_unstemmed | A comparison of E15.5 fetus and newborn rat serum proteomes |
title_short | A comparison of E15.5 fetus and newborn rat serum proteomes |
title_sort | comparison of e15.5 fetus and newborn rat serum proteomes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583134/ https://www.ncbi.nlm.nih.gov/pubmed/23134622 http://dx.doi.org/10.1186/1477-5956-10-64 |
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