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Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma

Microtubules play extensive roles in cellular processes, including cell motility. Stathmin is an important protein which destabilizes microtubules. The essential function of stathmin is closely associated with its phosphorylation status. Stathmin is overexpressed in many human cancers and has a sign...

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Detalles Bibliográficos
Autores principales: LIU, FEI, SUN, YU-LIN, XU, YANG, LIU, FANG, WANG, LI-SHUN, ZHAO, XIAO-HANG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583596/
https://www.ncbi.nlm.nih.gov/pubmed/23229199
http://dx.doi.org/10.3892/or.2012.2157
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author LIU, FEI
SUN, YU-LIN
XU, YANG
LIU, FANG
WANG, LI-SHUN
ZHAO, XIAO-HANG
author_facet LIU, FEI
SUN, YU-LIN
XU, YANG
LIU, FANG
WANG, LI-SHUN
ZHAO, XIAO-HANG
author_sort LIU, FEI
collection PubMed
description Microtubules play extensive roles in cellular processes, including cell motility. Stathmin is an important protein which destabilizes microtubules. The essential function of stathmin is closely associated with its phosphorylation status. Stathmin is overexpressed in many human cancers and has a significant relationship with clinical characteristics such as grade, tumor size and prognosis. We demonstrated that stathmin was overexpressed in ESCC tissues using both 2-DE and immunohistochemistry analysis. In addition, overexpression of stathmin was significantly correlated with histological grade in ESCC. However, no correlation was found with age, gender and lymph node metastasis. Knockdown of stathmin with siRNA impaired cell migration in KYSE30 and KYSE410 cells. When EC0156 cells were treated with paclitaxel, stathmin was stably phosphorylated and migration was impaired. These observations suggest that stathmin may have a more important function in ESCC development and migration. The present study provides further understanding of the importance of stathmin in ESCC therapy or diagnosis.
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spelling pubmed-35835962013-02-28 Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma LIU, FEI SUN, YU-LIN XU, YANG LIU, FANG WANG, LI-SHUN ZHAO, XIAO-HANG Oncol Rep Articles Microtubules play extensive roles in cellular processes, including cell motility. Stathmin is an important protein which destabilizes microtubules. The essential function of stathmin is closely associated with its phosphorylation status. Stathmin is overexpressed in many human cancers and has a significant relationship with clinical characteristics such as grade, tumor size and prognosis. We demonstrated that stathmin was overexpressed in ESCC tissues using both 2-DE and immunohistochemistry analysis. In addition, overexpression of stathmin was significantly correlated with histological grade in ESCC. However, no correlation was found with age, gender and lymph node metastasis. Knockdown of stathmin with siRNA impaired cell migration in KYSE30 and KYSE410 cells. When EC0156 cells were treated with paclitaxel, stathmin was stably phosphorylated and migration was impaired. These observations suggest that stathmin may have a more important function in ESCC development and migration. The present study provides further understanding of the importance of stathmin in ESCC therapy or diagnosis. D.A. Spandidos 2013-02 2012-11-28 /pmc/articles/PMC3583596/ /pubmed/23229199 http://dx.doi.org/10.3892/or.2012.2157 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
LIU, FEI
SUN, YU-LIN
XU, YANG
LIU, FANG
WANG, LI-SHUN
ZHAO, XIAO-HANG
Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma
title Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma
title_full Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma
title_fullStr Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma
title_full_unstemmed Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma
title_short Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma
title_sort expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583596/
https://www.ncbi.nlm.nih.gov/pubmed/23229199
http://dx.doi.org/10.3892/or.2012.2157
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