Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells

This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mech...

Descripción completa

Detalles Bibliográficos
Autores principales: BULJ, ZRINKA, DUCHI, SERENA, BEVILACQUA, ALESSANDRO, GHERARDI, ALESSANDRO, DOZZA, BARBARA, PICCININI, FILIPPO, MARIANI, GIULIA ADALGISA, LUCARELLI, ENRICO, GIANNINI, SANDRO, DONATI, DAVIDE, MARMIROLI, SANDRA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583637/
https://www.ncbi.nlm.nih.gov/pubmed/23165443
http://dx.doi.org/10.3892/ijo.2012.1700
Descripción
Sumario:This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.

Ejemplares similares