Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells

This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mech...

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Autores principales: BULJ, ZRINKA, DUCHI, SERENA, BEVILACQUA, ALESSANDRO, GHERARDI, ALESSANDRO, DOZZA, BARBARA, PICCININI, FILIPPO, MARIANI, GIULIA ADALGISA, LUCARELLI, ENRICO, GIANNINI, SANDRO, DONATI, DAVIDE, MARMIROLI, SANDRA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583637/
https://www.ncbi.nlm.nih.gov/pubmed/23165443
http://dx.doi.org/10.3892/ijo.2012.1700
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author BULJ, ZRINKA
DUCHI, SERENA
BEVILACQUA, ALESSANDRO
GHERARDI, ALESSANDRO
DOZZA, BARBARA
PICCININI, FILIPPO
MARIANI, GIULIA ADALGISA
LUCARELLI, ENRICO
GIANNINI, SANDRO
DONATI, DAVIDE
MARMIROLI, SANDRA
author_facet BULJ, ZRINKA
DUCHI, SERENA
BEVILACQUA, ALESSANDRO
GHERARDI, ALESSANDRO
DOZZA, BARBARA
PICCININI, FILIPPO
MARIANI, GIULIA ADALGISA
LUCARELLI, ENRICO
GIANNINI, SANDRO
DONATI, DAVIDE
MARMIROLI, SANDRA
author_sort BULJ, ZRINKA
collection PubMed
description This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.
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spelling pubmed-35836372013-03-04 Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells BULJ, ZRINKA DUCHI, SERENA BEVILACQUA, ALESSANDRO GHERARDI, ALESSANDRO DOZZA, BARBARA PICCININI, FILIPPO MARIANI, GIULIA ADALGISA LUCARELLI, ENRICO GIANNINI, SANDRO DONATI, DAVIDE MARMIROLI, SANDRA Int J Oncol Articles This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration. D.A. Spandidos 2012-11-15 /pmc/articles/PMC3583637/ /pubmed/23165443 http://dx.doi.org/10.3892/ijo.2012.1700 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
BULJ, ZRINKA
DUCHI, SERENA
BEVILACQUA, ALESSANDRO
GHERARDI, ALESSANDRO
DOZZA, BARBARA
PICCININI, FILIPPO
MARIANI, GIULIA ADALGISA
LUCARELLI, ENRICO
GIANNINI, SANDRO
DONATI, DAVIDE
MARMIROLI, SANDRA
Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
title Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
title_full Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
title_fullStr Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
title_full_unstemmed Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
title_short Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
title_sort protein kinase b/akt isoform 2 drives migration of human mesenchymal stem cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583637/
https://www.ncbi.nlm.nih.gov/pubmed/23165443
http://dx.doi.org/10.3892/ijo.2012.1700
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