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Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells

Rho GDP-dissociation inhibitors (RhoGDIs) are regulators of Rho family GTPases. RhoGDIβ has been implicated in cancer progression, but its precise role remains unclear. We determined the subcellular localization of RhoGDIβ and examined the effects of its overexpression and RNAi knockdown in cancer c...

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Autores principales: JIANG, YONG-SHENG, MAEDA, MASAYO, OKAMOTO, MAYUMI, FUJII, MIKIKO, FUKUTOMI, RYUICHIRO, HORI, MASATO, TATSUKA, MASAAKI, OTA, TAKAHIDE
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583720/
https://www.ncbi.nlm.nih.gov/pubmed/23232495
http://dx.doi.org/10.3892/ijo.2012.1730
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author JIANG, YONG-SHENG
MAEDA, MASAYO
OKAMOTO, MAYUMI
FUJII, MIKIKO
FUKUTOMI, RYUICHIRO
HORI, MASATO
TATSUKA, MASAAKI
OTA, TAKAHIDE
author_facet JIANG, YONG-SHENG
MAEDA, MASAYO
OKAMOTO, MAYUMI
FUJII, MIKIKO
FUKUTOMI, RYUICHIRO
HORI, MASATO
TATSUKA, MASAAKI
OTA, TAKAHIDE
author_sort JIANG, YONG-SHENG
collection PubMed
description Rho GDP-dissociation inhibitors (RhoGDIs) are regulators of Rho family GTPases. RhoGDIβ has been implicated in cancer progression, but its precise role remains unclear. We determined the subcellular localization of RhoGDIβ and examined the effects of its overexpression and RNAi knockdown in cancer cells. Immunofluorescence staining showed that RhoGDIβ localized to centrosomes in human cancer cells. In HeLa cells, exogenous GFP-tagged RhoGDIβ localized to centrosomes and its overexpression caused prolonged mitosis and aberrant cytokinesis in which the cell shape was distorted. RNAi knockdown of RhoGDIβ led to increased incidence of monopolar spindle mitosis resulting in polyploid cells. These results suggest that RhoGDIβ has mitotic functions, including regulation of cytokinesis and bipolar spindle formation. The dysregulated expression of RhoGDIβ may contribute to cancer progression by disrupting these processes.
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spelling pubmed-35837202013-03-04 Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells JIANG, YONG-SHENG MAEDA, MASAYO OKAMOTO, MAYUMI FUJII, MIKIKO FUKUTOMI, RYUICHIRO HORI, MASATO TATSUKA, MASAAKI OTA, TAKAHIDE Int J Oncol Articles Rho GDP-dissociation inhibitors (RhoGDIs) are regulators of Rho family GTPases. RhoGDIβ has been implicated in cancer progression, but its precise role remains unclear. We determined the subcellular localization of RhoGDIβ and examined the effects of its overexpression and RNAi knockdown in cancer cells. Immunofluorescence staining showed that RhoGDIβ localized to centrosomes in human cancer cells. In HeLa cells, exogenous GFP-tagged RhoGDIβ localized to centrosomes and its overexpression caused prolonged mitosis and aberrant cytokinesis in which the cell shape was distorted. RNAi knockdown of RhoGDIβ led to increased incidence of monopolar spindle mitosis resulting in polyploid cells. These results suggest that RhoGDIβ has mitotic functions, including regulation of cytokinesis and bipolar spindle formation. The dysregulated expression of RhoGDIβ may contribute to cancer progression by disrupting these processes. D.A. Spandidos 2012-12-07 /pmc/articles/PMC3583720/ /pubmed/23232495 http://dx.doi.org/10.3892/ijo.2012.1730 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
JIANG, YONG-SHENG
MAEDA, MASAYO
OKAMOTO, MAYUMI
FUJII, MIKIKO
FUKUTOMI, RYUICHIRO
HORI, MASATO
TATSUKA, MASAAKI
OTA, TAKAHIDE
Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells
title Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells
title_full Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells
title_fullStr Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells
title_full_unstemmed Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells
title_short Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells
title_sort centrosomal localization of rhogdiβ and its relevance to mitotic processes in cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583720/
https://www.ncbi.nlm.nih.gov/pubmed/23232495
http://dx.doi.org/10.3892/ijo.2012.1730
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