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Why and how protein aggregation has to be studied in vivo
The understanding of protein aggregation is a central issue in different fields of protein science, from the heterologous protein production in biotechnology to amyloid aggregation in several neurodegenerative and systemic diseases. To this goal, it became more and more evident the crucial relevance...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583745/ https://www.ncbi.nlm.nih.gov/pubmed/23410248 http://dx.doi.org/10.1186/1475-2859-12-17 |
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author | Ami, Diletta Natalello, Antonino Lotti, Marina Doglia, Silvia Maria |
author_facet | Ami, Diletta Natalello, Antonino Lotti, Marina Doglia, Silvia Maria |
author_sort | Ami, Diletta |
collection | PubMed |
description | The understanding of protein aggregation is a central issue in different fields of protein science, from the heterologous protein production in biotechnology to amyloid aggregation in several neurodegenerative and systemic diseases. To this goal, it became more and more evident the crucial relevance of studying protein aggregation in the complex cellular environment, since it allows to take into account the cellular components affecting protein aggregation, such as chaperones, proteases, and molecular crowding. Here, we discuss the use of several biochemical and biophysical approaches that can be employed to monitor protein aggregation within intact cells, focusing in particular on bacteria that are widely employed as microbial cell factories. |
format | Online Article Text |
id | pubmed-3583745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35837452013-02-28 Why and how protein aggregation has to be studied in vivo Ami, Diletta Natalello, Antonino Lotti, Marina Doglia, Silvia Maria Microb Cell Fact Commentary The understanding of protein aggregation is a central issue in different fields of protein science, from the heterologous protein production in biotechnology to amyloid aggregation in several neurodegenerative and systemic diseases. To this goal, it became more and more evident the crucial relevance of studying protein aggregation in the complex cellular environment, since it allows to take into account the cellular components affecting protein aggregation, such as chaperones, proteases, and molecular crowding. Here, we discuss the use of several biochemical and biophysical approaches that can be employed to monitor protein aggregation within intact cells, focusing in particular on bacteria that are widely employed as microbial cell factories. BioMed Central 2013-02-15 /pmc/articles/PMC3583745/ /pubmed/23410248 http://dx.doi.org/10.1186/1475-2859-12-17 Text en Copyright ©2013 Ami et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Commentary Ami, Diletta Natalello, Antonino Lotti, Marina Doglia, Silvia Maria Why and how protein aggregation has to be studied in vivo |
title | Why and how protein aggregation has to be studied in vivo |
title_full | Why and how protein aggregation has to be studied in vivo |
title_fullStr | Why and how protein aggregation has to be studied in vivo |
title_full_unstemmed | Why and how protein aggregation has to be studied in vivo |
title_short | Why and how protein aggregation has to be studied in vivo |
title_sort | why and how protein aggregation has to be studied in vivo |
topic | Commentary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583745/ https://www.ncbi.nlm.nih.gov/pubmed/23410248 http://dx.doi.org/10.1186/1475-2859-12-17 |
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