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The effects of ghrelin on colonic anastomosis healing in rats

OBJECTIVES: In addition to its roles in the stimulation of growth hormone secretion and the regulation of appetite and metabolism, ghrelin exerts immunomodulatory, anti-inflammatory and antioxidant actions in several organ systems. In this study, we investigated the effects of ghrelin on the healing...

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Autores principales: Ceran, Canan, Aksoy, Rauf Tuğrul, Gülbahar, Özlem, Öztürk, Figen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584276/
https://www.ncbi.nlm.nih.gov/pubmed/23525322
http://dx.doi.org/10.6061/clinics/2013(02)OA19
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author Ceran, Canan
Aksoy, Rauf Tuğrul
Gülbahar, Özlem
Öztürk, Figen
author_facet Ceran, Canan
Aksoy, Rauf Tuğrul
Gülbahar, Özlem
Öztürk, Figen
author_sort Ceran, Canan
collection PubMed
description OBJECTIVES: In addition to its roles in the stimulation of growth hormone secretion and the regulation of appetite and metabolism, ghrelin exerts immunomodulatory, anti-inflammatory and antioxidant actions in several organ systems. In this study, we investigated the effects of ghrelin on the healing of experimental colonic anastomoses. METHODS: Wistar rats were randomly divided into two groups (n = 10 in each). A segment of colon was excised, and an end-to-end anastomosis was performed in the distal colon. The Ghrelin Group received 10 ng/kg/day IP ghrelin for seven days postoperatively, whereas the Control Group received an identical volume of saline. On the seventh postoperative day, the anastomotic bursting pressures and hydroxyproline levels were measured, and adhesion formation around the anastomoses was examined. Histopathological analyses were performed to evaluate inflammatory cell infiltration, fibroblast infiltration, collagen density and neovascularization. RESULTS: In the Ghrelin Group, the bursting pressure and hydroxyproline levels were significantly higher than in the Control Group. The adhesion formation scores were lower in the Ghrelin Group than in the Control Group. Although the inflammatory cell infiltration was diminished in the Ghrelin Group, the degrees of fibroblast infiltration, collagen density and neovascularization were not significantly different between the groups. CONCLUSION: Our results indicate that ghrelin improves the healing of colonic anastomoses in rats.
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spelling pubmed-35842762013-03-01 The effects of ghrelin on colonic anastomosis healing in rats Ceran, Canan Aksoy, Rauf Tuğrul Gülbahar, Özlem Öztürk, Figen Clinics (Sao Paulo) Basic Research OBJECTIVES: In addition to its roles in the stimulation of growth hormone secretion and the regulation of appetite and metabolism, ghrelin exerts immunomodulatory, anti-inflammatory and antioxidant actions in several organ systems. In this study, we investigated the effects of ghrelin on the healing of experimental colonic anastomoses. METHODS: Wistar rats were randomly divided into two groups (n = 10 in each). A segment of colon was excised, and an end-to-end anastomosis was performed in the distal colon. The Ghrelin Group received 10 ng/kg/day IP ghrelin for seven days postoperatively, whereas the Control Group received an identical volume of saline. On the seventh postoperative day, the anastomotic bursting pressures and hydroxyproline levels were measured, and adhesion formation around the anastomoses was examined. Histopathological analyses were performed to evaluate inflammatory cell infiltration, fibroblast infiltration, collagen density and neovascularization. RESULTS: In the Ghrelin Group, the bursting pressure and hydroxyproline levels were significantly higher than in the Control Group. The adhesion formation scores were lower in the Ghrelin Group than in the Control Group. Although the inflammatory cell infiltration was diminished in the Ghrelin Group, the degrees of fibroblast infiltration, collagen density and neovascularization were not significantly different between the groups. CONCLUSION: Our results indicate that ghrelin improves the healing of colonic anastomoses in rats. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2013-02 /pmc/articles/PMC3584276/ /pubmed/23525322 http://dx.doi.org/10.6061/clinics/2013(02)OA19 Text en Copyright © 2013 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Ceran, Canan
Aksoy, Rauf Tuğrul
Gülbahar, Özlem
Öztürk, Figen
The effects of ghrelin on colonic anastomosis healing in rats
title The effects of ghrelin on colonic anastomosis healing in rats
title_full The effects of ghrelin on colonic anastomosis healing in rats
title_fullStr The effects of ghrelin on colonic anastomosis healing in rats
title_full_unstemmed The effects of ghrelin on colonic anastomosis healing in rats
title_short The effects of ghrelin on colonic anastomosis healing in rats
title_sort effects of ghrelin on colonic anastomosis healing in rats
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584276/
https://www.ncbi.nlm.nih.gov/pubmed/23525322
http://dx.doi.org/10.6061/clinics/2013(02)OA19
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