Cargando…

Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay

OBJECTIVE: In this study, we investigated the combined effect of 2-Methoxyestradiol (2ME2) and (60)Co on the cytogenetic damage of iododeoxyuridine (IUdR) in the spheroid model of U87MG glioblastoma cancer cell lines by alkaline comet assay. MATERIALS AND METHODS: 1. Vehicle (this sample was not tre...

Descripción completa

Detalles Bibliográficos
Autores principales: Khoei, Samideh, Delfan, Sara, Neshasteh-Riz, Ali, Mahdavi, Seyed Rabi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584462/
https://www.ncbi.nlm.nih.gov/pubmed/23508289
_version_ 1782261024488947712
author Khoei, Samideh
Delfan, Sara
Neshasteh-Riz, Ali
Mahdavi, Seyed Rabi
author_facet Khoei, Samideh
Delfan, Sara
Neshasteh-Riz, Ali
Mahdavi, Seyed Rabi
author_sort Khoei, Samideh
collection PubMed
description OBJECTIVE: In this study, we investigated the combined effect of 2-Methoxyestradiol (2ME2) and (60)Co on the cytogenetic damage of iododeoxyuridine (IUdR) in the spheroid model of U87MG glioblastoma cancer cell lines by alkaline comet assay. MATERIALS AND METHODS: 1. Vehicle (this sample was not treated in the 2(nd) VDT). 2. Treated with 2ME2 (250 µM) for 1 VDT. 3. Treated simultaneously with 2ME2 (250 µM) and IUdR (1 µM) for 1 VDT. 4. Treated with 2ME2 (250 µM) for 1 VDT then irradiated with (60)Co (2 Gy). 5. Treated simultaneously with 2ME2 (250 µM) and IUdR (1 µM) for 1 VDT then irradiated with (60)Co (2 Gy). Then the DNA damage was evaluated using the alkaline comet assay method. RESULTS: The results showed that 2ME2 in combination with gamma irradiation of (60)Co significantly (p<0.001) increased the DNA damage by IUdR as compared to the control group. Thus the combination of these two agents increased the cytogenetic effects of IUdR in the spheroid culture model of U87MG glioblastoma cell lines. CONCLUSION: By inhibiting the HIF-1α protein and preventing the G(0) phase arrest, 2ME2 causes an increased progression into S phase and increases the IUdR absorption. Then the DNA damage in the spheroid cells increases as the uptake of IUdR is increased. These results suggest that the combined use of 2ME2 and (60)Co can increase the radiosensitization effect of IUdR.
format Online
Article
Text
id pubmed-3584462
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Royan Institute
record_format MEDLINE/PubMed
spelling pubmed-35844622013-03-18 Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay Khoei, Samideh Delfan, Sara Neshasteh-Riz, Ali Mahdavi, Seyed Rabi Cell J Research Article OBJECTIVE: In this study, we investigated the combined effect of 2-Methoxyestradiol (2ME2) and (60)Co on the cytogenetic damage of iododeoxyuridine (IUdR) in the spheroid model of U87MG glioblastoma cancer cell lines by alkaline comet assay. MATERIALS AND METHODS: 1. Vehicle (this sample was not treated in the 2(nd) VDT). 2. Treated with 2ME2 (250 µM) for 1 VDT. 3. Treated simultaneously with 2ME2 (250 µM) and IUdR (1 µM) for 1 VDT. 4. Treated with 2ME2 (250 µM) for 1 VDT then irradiated with (60)Co (2 Gy). 5. Treated simultaneously with 2ME2 (250 µM) and IUdR (1 µM) for 1 VDT then irradiated with (60)Co (2 Gy). Then the DNA damage was evaluated using the alkaline comet assay method. RESULTS: The results showed that 2ME2 in combination with gamma irradiation of (60)Co significantly (p<0.001) increased the DNA damage by IUdR as compared to the control group. Thus the combination of these two agents increased the cytogenetic effects of IUdR in the spheroid culture model of U87MG glioblastoma cell lines. CONCLUSION: By inhibiting the HIF-1α protein and preventing the G(0) phase arrest, 2ME2 causes an increased progression into S phase and increases the IUdR absorption. Then the DNA damage in the spheroid cells increases as the uptake of IUdR is increased. These results suggest that the combined use of 2ME2 and (60)Co can increase the radiosensitization effect of IUdR. Royan Institute 2011 2011-08-24 /pmc/articles/PMC3584462/ /pubmed/23508289 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Khoei, Samideh
Delfan, Sara
Neshasteh-Riz, Ali
Mahdavi, Seyed Rabi
Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay
title Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay
title_full Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay
title_fullStr Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay
title_full_unstemmed Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay
title_short Evaluation of the Combined Effect of 2ME2 and (60)Co on the Inducement of DNA Damage by IUdR in a Spheroid Model of the U87MG Glioblastoma Cancer Cell Line Using Alkaline Comet Assay
title_sort evaluation of the combined effect of 2me2 and (60)co on the inducement of dna damage by iudr in a spheroid model of the u87mg glioblastoma cancer cell line using alkaline comet assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584462/
https://www.ncbi.nlm.nih.gov/pubmed/23508289
work_keys_str_mv AT khoeisamideh evaluationofthecombinedeffectof2me2and60coontheinducementofdnadamagebyiudrinaspheroidmodeloftheu87mgglioblastomacancercelllineusingalkalinecometassay
AT delfansara evaluationofthecombinedeffectof2me2and60coontheinducementofdnadamagebyiudrinaspheroidmodeloftheu87mgglioblastomacancercelllineusingalkalinecometassay
AT neshastehrizali evaluationofthecombinedeffectof2me2and60coontheinducementofdnadamagebyiudrinaspheroidmodeloftheu87mgglioblastomacancercelllineusingalkalinecometassay
AT mahdaviseyedrabi evaluationofthecombinedeffectof2me2and60coontheinducementofdnadamagebyiudrinaspheroidmodeloftheu87mgglioblastomacancercelllineusingalkalinecometassay