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Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents

AIM: To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO). METHODS: Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5...

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Autores principales: Dobrivojević, Marina, Bohaček, Ivan, Erjavec, Igor, Gorup, Dunja, Gajović, Srećko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Medical Schools 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584505/
https://www.ncbi.nlm.nih.gov/pubmed/23444240
http://dx.doi.org/10.3325/cmj.2013.54.3
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author Dobrivojević, Marina
Bohaček, Ivan
Erjavec, Igor
Gorup, Dunja
Gajović, Srećko
author_facet Dobrivojević, Marina
Bohaček, Ivan
Erjavec, Igor
Gorup, Dunja
Gajović, Srećko
author_sort Dobrivojević, Marina
collection PubMed
description AIM: To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO). METHODS: Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed. RESULTS: Nonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity. CONCLUSION: MicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema.
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spelling pubmed-35845052013-02-28 Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents Dobrivojević, Marina Bohaček, Ivan Erjavec, Igor Gorup, Dunja Gajović, Srećko Croat Med J Clinical Science AIM: To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO). METHODS: Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed. RESULTS: Nonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity. CONCLUSION: MicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema. Croatian Medical Schools 2013-02 /pmc/articles/PMC3584505/ /pubmed/23444240 http://dx.doi.org/10.3325/cmj.2013.54.3 Text en Copyright © 2013 by the Croatian Medical Journal. All rights reserved. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Science
Dobrivojević, Marina
Bohaček, Ivan
Erjavec, Igor
Gorup, Dunja
Gajović, Srećko
Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
title Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
title_full Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
title_fullStr Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
title_full_unstemmed Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
title_short Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
title_sort computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
topic Clinical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584505/
https://www.ncbi.nlm.nih.gov/pubmed/23444240
http://dx.doi.org/10.3325/cmj.2013.54.3
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