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Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
AIM: To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO). METHODS: Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Croatian Medical Schools
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584505/ https://www.ncbi.nlm.nih.gov/pubmed/23444240 http://dx.doi.org/10.3325/cmj.2013.54.3 |
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author | Dobrivojević, Marina Bohaček, Ivan Erjavec, Igor Gorup, Dunja Gajović, Srećko |
author_facet | Dobrivojević, Marina Bohaček, Ivan Erjavec, Igor Gorup, Dunja Gajović, Srećko |
author_sort | Dobrivojević, Marina |
collection | PubMed |
description | AIM: To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO). METHODS: Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed. RESULTS: Nonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity. CONCLUSION: MicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema. |
format | Online Article Text |
id | pubmed-3584505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Croatian Medical Schools |
record_format | MEDLINE/PubMed |
spelling | pubmed-35845052013-02-28 Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents Dobrivojević, Marina Bohaček, Ivan Erjavec, Igor Gorup, Dunja Gajović, Srećko Croat Med J Clinical Science AIM: To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO). METHODS: Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed. RESULTS: Nonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity. CONCLUSION: MicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema. Croatian Medical Schools 2013-02 /pmc/articles/PMC3584505/ /pubmed/23444240 http://dx.doi.org/10.3325/cmj.2013.54.3 Text en Copyright © 2013 by the Croatian Medical Journal. All rights reserved. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Science Dobrivojević, Marina Bohaček, Ivan Erjavec, Igor Gorup, Dunja Gajović, Srećko Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
title | Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
title_full | Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
title_fullStr | Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
title_full_unstemmed | Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
title_short | Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
title_sort | computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents |
topic | Clinical Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584505/ https://www.ncbi.nlm.nih.gov/pubmed/23444240 http://dx.doi.org/10.3325/cmj.2013.54.3 |
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