Cargando…

Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum

Epigenetic mechanisms are emerging as one of the major factors of the dynamics of gene expression in the human malaria parasite, Plasmodium falciparum. To elucidate the role of chromatin remodeling in transcriptional regulation associated with the progression of the P. falciparum intraerythrocytic d...

Descripción completa

Detalles Bibliográficos
Autores principales: Gupta, Archna P., Chin, Wai Hoe, Zhu, Lei, Mok, Sachel, Luah, Yen-Hoon, Lim, Eng-How, Bozdech, Zbynek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585154/
https://www.ncbi.nlm.nih.gov/pubmed/23468622
http://dx.doi.org/10.1371/journal.ppat.1003170
_version_ 1782261108746223616
author Gupta, Archna P.
Chin, Wai Hoe
Zhu, Lei
Mok, Sachel
Luah, Yen-Hoon
Lim, Eng-How
Bozdech, Zbynek
author_facet Gupta, Archna P.
Chin, Wai Hoe
Zhu, Lei
Mok, Sachel
Luah, Yen-Hoon
Lim, Eng-How
Bozdech, Zbynek
author_sort Gupta, Archna P.
collection PubMed
description Epigenetic mechanisms are emerging as one of the major factors of the dynamics of gene expression in the human malaria parasite, Plasmodium falciparum. To elucidate the role of chromatin remodeling in transcriptional regulation associated with the progression of the P. falciparum intraerythrocytic development cycle (IDC), we mapped the temporal pattern of chromosomal association with histone H3 and H4 modifications using ChIP-on-chip. Here, we have generated a broad integrative epigenomic map of twelve histone modifications during the P. falciparum IDC including H4K5ac, H4K8ac, H4K12ac, H4K16ac, H3K9ac, H3K14ac, H3K56ac, H4K20me1, H4K20me3, H3K4me3, H3K79me3 and H4R3me2. While some modifications were found to be associated with the vast majority of the genome and their occupancy was constant, others showed more specific and highly dynamic distribution. Importantly, eight modifications displaying tight correlations with transcript levels showed differential affinity to distinct genomic regions with H4K8ac occupying predominantly promoter regions while others occurred at the 5′ ends of coding sequences. The promoter occupancy of H4K8ac remained unchanged when ectopically inserted at a different locus, indicating the presence of specific DNA elements that recruit histone modifying enzymes regardless of their broad chromatin environment. In addition, we showed the presence of multivalent domains on the genome carrying more than one histone mark, highlighting the importance of combinatorial effects on transcription. Overall, our work portrays a substantial association between chromosomal locations of various epigenetic markers, transcriptional activity and global stage-specific transitions in the epigenome.
format Online
Article
Text
id pubmed-3585154
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35851542013-03-06 Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum Gupta, Archna P. Chin, Wai Hoe Zhu, Lei Mok, Sachel Luah, Yen-Hoon Lim, Eng-How Bozdech, Zbynek PLoS Pathog Research Article Epigenetic mechanisms are emerging as one of the major factors of the dynamics of gene expression in the human malaria parasite, Plasmodium falciparum. To elucidate the role of chromatin remodeling in transcriptional regulation associated with the progression of the P. falciparum intraerythrocytic development cycle (IDC), we mapped the temporal pattern of chromosomal association with histone H3 and H4 modifications using ChIP-on-chip. Here, we have generated a broad integrative epigenomic map of twelve histone modifications during the P. falciparum IDC including H4K5ac, H4K8ac, H4K12ac, H4K16ac, H3K9ac, H3K14ac, H3K56ac, H4K20me1, H4K20me3, H3K4me3, H3K79me3 and H4R3me2. While some modifications were found to be associated with the vast majority of the genome and their occupancy was constant, others showed more specific and highly dynamic distribution. Importantly, eight modifications displaying tight correlations with transcript levels showed differential affinity to distinct genomic regions with H4K8ac occupying predominantly promoter regions while others occurred at the 5′ ends of coding sequences. The promoter occupancy of H4K8ac remained unchanged when ectopically inserted at a different locus, indicating the presence of specific DNA elements that recruit histone modifying enzymes regardless of their broad chromatin environment. In addition, we showed the presence of multivalent domains on the genome carrying more than one histone mark, highlighting the importance of combinatorial effects on transcription. Overall, our work portrays a substantial association between chromosomal locations of various epigenetic markers, transcriptional activity and global stage-specific transitions in the epigenome. Public Library of Science 2013-02-28 /pmc/articles/PMC3585154/ /pubmed/23468622 http://dx.doi.org/10.1371/journal.ppat.1003170 Text en © 2013 Gupta et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gupta, Archna P.
Chin, Wai Hoe
Zhu, Lei
Mok, Sachel
Luah, Yen-Hoon
Lim, Eng-How
Bozdech, Zbynek
Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum
title Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum
title_full Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum
title_fullStr Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum
title_full_unstemmed Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum
title_short Dynamic Epigenetic Regulation of Gene Expression during the Life Cycle of Malaria Parasite Plasmodium falciparum
title_sort dynamic epigenetic regulation of gene expression during the life cycle of malaria parasite plasmodium falciparum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585154/
https://www.ncbi.nlm.nih.gov/pubmed/23468622
http://dx.doi.org/10.1371/journal.ppat.1003170
work_keys_str_mv AT guptaarchnap dynamicepigeneticregulationofgeneexpressionduringthelifecycleofmalariaparasiteplasmodiumfalciparum
AT chinwaihoe dynamicepigeneticregulationofgeneexpressionduringthelifecycleofmalariaparasiteplasmodiumfalciparum
AT zhulei dynamicepigeneticregulationofgeneexpressionduringthelifecycleofmalariaparasiteplasmodiumfalciparum
AT moksachel dynamicepigeneticregulationofgeneexpressionduringthelifecycleofmalariaparasiteplasmodiumfalciparum
AT luahyenhoon dynamicepigeneticregulationofgeneexpressionduringthelifecycleofmalariaparasiteplasmodiumfalciparum
AT limenghow dynamicepigeneticregulationofgeneexpressionduringthelifecycleofmalariaparasiteplasmodiumfalciparum
AT bozdechzbynek dynamicepigeneticregulationofgeneexpressionduringthelifecycleofmalariaparasiteplasmodiumfalciparum