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Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells

Somatic cell nuclear transfer (SCNT) is an important method of breeding quality varieties, expanding groups, and preserving endangered species. However, the viability of SCNT embryos is poor, and the cloned rate of animal production is low in pig. This study aims to investigate the gene function and...

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Autores principales: Wei, Hongjiang, Qing, Yubo, Pan, Weirong, Zhao, Hongye, Li, Honghui, Cheng, Wenmin, Zhao, Lu, Xu, Chengsheng, Li, Hong, Li, Si, Ye, Lei, Wei, Taiyun, Li, Xiaobing, Fu, Guowen, Li, Wengui, Xin, Jige, Zeng, Yangzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585185/
https://www.ncbi.nlm.nih.gov/pubmed/23469059
http://dx.doi.org/10.1371/journal.pone.0057728
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author Wei, Hongjiang
Qing, Yubo
Pan, Weirong
Zhao, Hongye
Li, Honghui
Cheng, Wenmin
Zhao, Lu
Xu, Chengsheng
Li, Hong
Li, Si
Ye, Lei
Wei, Taiyun
Li, Xiaobing
Fu, Guowen
Li, Wengui
Xin, Jige
Zeng, Yangzhi
author_facet Wei, Hongjiang
Qing, Yubo
Pan, Weirong
Zhao, Hongye
Li, Honghui
Cheng, Wenmin
Zhao, Lu
Xu, Chengsheng
Li, Hong
Li, Si
Ye, Lei
Wei, Taiyun
Li, Xiaobing
Fu, Guowen
Li, Wengui
Xin, Jige
Zeng, Yangzhi
author_sort Wei, Hongjiang
collection PubMed
description Somatic cell nuclear transfer (SCNT) is an important method of breeding quality varieties, expanding groups, and preserving endangered species. However, the viability of SCNT embryos is poor, and the cloned rate of animal production is low in pig. This study aims to investigate the gene function and establish a disease model of Banna miniature inbred pig. SCNT with donor cells derived from fetal, newborn, and adult fibroblasts was performed, and the cloning efficiencies among the donor cells were compared. The results showed that the cleavage and blastocyst formation rates did not significantly differ between the reconstructed embryos derived from the fetal (74.3% and 27.4%) and newborn (76.4% and 21.8%) fibroblasts of the Banna miniature inbred pig (P>0.05). However, both fetal and newborn fibroblast groups showed significantly higher rates than the adult fibroblast group (61.9% and 13.0%; P<0.05). The pregnancy rates of the recipients in the fetal and newborn fibroblast groups (60% and 80%, respectively) were higher than those in the adult fibroblast group. Eight, three, and one cloned piglet were obtained from reconstructed embryos of the fetal, newborn, and adult fibroblasts, respectively. Microsatellite analyses results indicated that the genotypes of all cloning piglets were identical to their donor cells and that the genetic homozygosity of the Banna miniature inbred pig was higher than those of the recipients. Therefore, the offspring was successfully cloned using the fetal, newborn, and adult fibroblasts of Banna miniature inbred pig as donor cells.
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spelling pubmed-35851852013-03-06 Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells Wei, Hongjiang Qing, Yubo Pan, Weirong Zhao, Hongye Li, Honghui Cheng, Wenmin Zhao, Lu Xu, Chengsheng Li, Hong Li, Si Ye, Lei Wei, Taiyun Li, Xiaobing Fu, Guowen Li, Wengui Xin, Jige Zeng, Yangzhi PLoS One Research Article Somatic cell nuclear transfer (SCNT) is an important method of breeding quality varieties, expanding groups, and preserving endangered species. However, the viability of SCNT embryos is poor, and the cloned rate of animal production is low in pig. This study aims to investigate the gene function and establish a disease model of Banna miniature inbred pig. SCNT with donor cells derived from fetal, newborn, and adult fibroblasts was performed, and the cloning efficiencies among the donor cells were compared. The results showed that the cleavage and blastocyst formation rates did not significantly differ between the reconstructed embryos derived from the fetal (74.3% and 27.4%) and newborn (76.4% and 21.8%) fibroblasts of the Banna miniature inbred pig (P>0.05). However, both fetal and newborn fibroblast groups showed significantly higher rates than the adult fibroblast group (61.9% and 13.0%; P<0.05). The pregnancy rates of the recipients in the fetal and newborn fibroblast groups (60% and 80%, respectively) were higher than those in the adult fibroblast group. Eight, three, and one cloned piglet were obtained from reconstructed embryos of the fetal, newborn, and adult fibroblasts, respectively. Microsatellite analyses results indicated that the genotypes of all cloning piglets were identical to their donor cells and that the genetic homozygosity of the Banna miniature inbred pig was higher than those of the recipients. Therefore, the offspring was successfully cloned using the fetal, newborn, and adult fibroblasts of Banna miniature inbred pig as donor cells. Public Library of Science 2013-02-28 /pmc/articles/PMC3585185/ /pubmed/23469059 http://dx.doi.org/10.1371/journal.pone.0057728 Text en © 2013 Wei et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wei, Hongjiang
Qing, Yubo
Pan, Weirong
Zhao, Hongye
Li, Honghui
Cheng, Wenmin
Zhao, Lu
Xu, Chengsheng
Li, Hong
Li, Si
Ye, Lei
Wei, Taiyun
Li, Xiaobing
Fu, Guowen
Li, Wengui
Xin, Jige
Zeng, Yangzhi
Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells
title Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells
title_full Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells
title_fullStr Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells
title_full_unstemmed Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells
title_short Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells
title_sort comparison of the efficiency of banna miniature inbred pig somatic cell nuclear transfer among different donor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585185/
https://www.ncbi.nlm.nih.gov/pubmed/23469059
http://dx.doi.org/10.1371/journal.pone.0057728
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