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Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae)
BACKGROUND: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of D...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585292/ https://www.ncbi.nlm.nih.gov/pubmed/23468937 http://dx.doi.org/10.1371/journal.pone.0057213 |
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author | Meng, Xiang Hu, Junjie Xu, Xiaoxia Wang, Zeqing Hu, Qiongbu Jin, Fengliang Ren, Shunxiang |
author_facet | Meng, Xiang Hu, Junjie Xu, Xiaoxia Wang, Zeqing Hu, Qiongbu Jin, Fengliang Ren, Shunxiang |
author_sort | Meng, Xiang |
collection | PubMed |
description | BACKGROUND: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out. PRINCIPAL FINDINGS: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment. CONCLUSIONS: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression. |
format | Online Article Text |
id | pubmed-3585292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35852922013-03-06 Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Meng, Xiang Hu, Junjie Xu, Xiaoxia Wang, Zeqing Hu, Qiongbu Jin, Fengliang Ren, Shunxiang PLoS One Research Article BACKGROUND: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out. PRINCIPAL FINDINGS: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment. CONCLUSIONS: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression. Public Library of Science 2013-02-28 /pmc/articles/PMC3585292/ /pubmed/23468937 http://dx.doi.org/10.1371/journal.pone.0057213 Text en © 2013 Meng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Meng, Xiang Hu, Junjie Xu, Xiaoxia Wang, Zeqing Hu, Qiongbu Jin, Fengliang Ren, Shunxiang Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) |
title | Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) |
title_full | Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) |
title_fullStr | Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) |
title_full_unstemmed | Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) |
title_short | Toxic Effect of Destruxin A on Abnormal Wing Disc-Like (SLAWD) in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) |
title_sort | toxic effect of destruxin a on abnormal wing disc-like (slawd) in spodoptera litura fabricius (lepidoptera: noctuidae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585292/ https://www.ncbi.nlm.nih.gov/pubmed/23468937 http://dx.doi.org/10.1371/journal.pone.0057213 |
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