Cargando…
Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses
Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H(2)O(2) into H(2)O and O(2), play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585366/ https://www.ncbi.nlm.nih.gov/pubmed/23468992 http://dx.doi.org/10.1371/journal.pone.0057472 |
_version_ | 1782261156836016128 |
---|---|
author | Zhang, Zhiguo Zhang, Quian Wu, Jinxia Zheng, Xia Zheng, Sheng Sun, Xuehui Qiu, Quansheng Lu, Tiegang |
author_facet | Zhang, Zhiguo Zhang, Quian Wu, Jinxia Zheng, Xia Zheng, Sheng Sun, Xuehui Qiu, Quansheng Lu, Tiegang |
author_sort | Zhang, Zhiguo |
collection | PubMed |
description | Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H(2)O(2) into H(2)O and O(2), play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H(2)O(2) and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species. |
format | Online Article Text |
id | pubmed-3585366 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35853662013-03-06 Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses Zhang, Zhiguo Zhang, Quian Wu, Jinxia Zheng, Xia Zheng, Sheng Sun, Xuehui Qiu, Quansheng Lu, Tiegang PLoS One Research Article Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H(2)O(2) into H(2)O and O(2), play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H(2)O(2) and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species. Public Library of Science 2013-02-28 /pmc/articles/PMC3585366/ /pubmed/23468992 http://dx.doi.org/10.1371/journal.pone.0057472 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Zhiguo Zhang, Quian Wu, Jinxia Zheng, Xia Zheng, Sheng Sun, Xuehui Qiu, Quansheng Lu, Tiegang Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses |
title | Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses |
title_full | Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses |
title_fullStr | Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses |
title_full_unstemmed | Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses |
title_short | Gene Knockout Study Reveals That Cytosolic Ascorbate Peroxidase 2(OsAPX2) Plays a Critical Role in Growth and Reproduction in Rice under Drought, Salt and Cold Stresses |
title_sort | gene knockout study reveals that cytosolic ascorbate peroxidase 2(osapx2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585366/ https://www.ncbi.nlm.nih.gov/pubmed/23468992 http://dx.doi.org/10.1371/journal.pone.0057472 |
work_keys_str_mv | AT zhangzhiguo geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses AT zhangquian geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses AT wujinxia geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses AT zhengxia geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses AT zhengsheng geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses AT sunxuehui geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses AT qiuquansheng geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses AT lutiegang geneknockoutstudyrevealsthatcytosolicascorbateperoxidase2osapx2playsacriticalroleingrowthandreproductioninriceunderdroughtsaltandcoldstresses |