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Unexpected Diversity during Community Succession in the Apple Flower Microbiome

Despite its importance to the host, the flower microbiome is poorly understood. We report a culture-independent, community-level assessment of apple flower microbial diversity and dynamics. We collected flowers from six apple trees at five time points, starting before flowers opened and ending at pe...

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Autores principales: Shade, Ashley, McManus, Patricia S., Handelsman, Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585449/
https://www.ncbi.nlm.nih.gov/pubmed/23443006
http://dx.doi.org/10.1128/mBio.00602-12
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author Shade, Ashley
McManus, Patricia S.
Handelsman, Jo
author_facet Shade, Ashley
McManus, Patricia S.
Handelsman, Jo
author_sort Shade, Ashley
collection PubMed
description Despite its importance to the host, the flower microbiome is poorly understood. We report a culture-independent, community-level assessment of apple flower microbial diversity and dynamics. We collected flowers from six apple trees at five time points, starting before flowers opened and ending at petal fall. We applied streptomycin to half of the trees when flowers opened. Assessment of microbial diversity using tag pyrosequencing of 16S rRNA genes revealed that the apple flower communities were rich and diverse and dominated by members of TM7 and Deinococcus-Thermus, phyla about which relatively little is known. From thousands of taxa, we identified six successional groups with coherent dynamics whose abundances peaked at different times before and after bud opening. We designated the groups Pioneer, Early, Mid, Late, Climax, and Generalist communities. The successional pattern was attributed to a set of prevalent taxa that were persistent and gradually changing in abundance. These taxa had significant associations with other community members, as demonstrated with a cooccurrence network based on local similarity analysis. We also detected a set of less-abundant, transient taxa that contributed to general tree-to-tree variability but not to the successional pattern. Communities on trees sprayed with streptomycin had slightly lower phylogenetic diversity than those on unsprayed trees but did not differ in structure or succession. Our results suggest that changes in apple flower microbial community structure are predictable over the life of the flower, providing a basis for ecological understanding and disease management.
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spelling pubmed-35854492013-03-05 Unexpected Diversity during Community Succession in the Apple Flower Microbiome Shade, Ashley McManus, Patricia S. Handelsman, Jo mBio Research Article Despite its importance to the host, the flower microbiome is poorly understood. We report a culture-independent, community-level assessment of apple flower microbial diversity and dynamics. We collected flowers from six apple trees at five time points, starting before flowers opened and ending at petal fall. We applied streptomycin to half of the trees when flowers opened. Assessment of microbial diversity using tag pyrosequencing of 16S rRNA genes revealed that the apple flower communities were rich and diverse and dominated by members of TM7 and Deinococcus-Thermus, phyla about which relatively little is known. From thousands of taxa, we identified six successional groups with coherent dynamics whose abundances peaked at different times before and after bud opening. We designated the groups Pioneer, Early, Mid, Late, Climax, and Generalist communities. The successional pattern was attributed to a set of prevalent taxa that were persistent and gradually changing in abundance. These taxa had significant associations with other community members, as demonstrated with a cooccurrence network based on local similarity analysis. We also detected a set of less-abundant, transient taxa that contributed to general tree-to-tree variability but not to the successional pattern. Communities on trees sprayed with streptomycin had slightly lower phylogenetic diversity than those on unsprayed trees but did not differ in structure or succession. Our results suggest that changes in apple flower microbial community structure are predictable over the life of the flower, providing a basis for ecological understanding and disease management. American Society of Microbiology 2013-02-26 /pmc/articles/PMC3585449/ /pubmed/23443006 http://dx.doi.org/10.1128/mBio.00602-12 Text en Copyright © 2013 Shade et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported (http://creativecommons.org/licenses/by-nc-sa/3.0/) license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shade, Ashley
McManus, Patricia S.
Handelsman, Jo
Unexpected Diversity during Community Succession in the Apple Flower Microbiome
title Unexpected Diversity during Community Succession in the Apple Flower Microbiome
title_full Unexpected Diversity during Community Succession in the Apple Flower Microbiome
title_fullStr Unexpected Diversity during Community Succession in the Apple Flower Microbiome
title_full_unstemmed Unexpected Diversity during Community Succession in the Apple Flower Microbiome
title_short Unexpected Diversity during Community Succession in the Apple Flower Microbiome
title_sort unexpected diversity during community succession in the apple flower microbiome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585449/
https://www.ncbi.nlm.nih.gov/pubmed/23443006
http://dx.doi.org/10.1128/mBio.00602-12
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