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Visualizing the Prostate Gland by MR Imaging in Young and Old Mice

PURPOSE: Prostate imaging requires optimization in young and old mouse models. We tested which MR sequences and field strengths best depict the prostate gland in young and old mice; and, whether prostate MR signal, size, and architecture change with age. TECHNIQUE: Magnetic resonance imaging (MRI) o...

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Autores principales: Ravoori, Murali, Duggal, Jyoti, Gagea, Mihai, Han, Lin, Singh, Sheela, Liu, Ping, Wei, Wei, Ragan, Dustin K., Bankson, James A., Ma, Jingfei, Kundra, Vikas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585879/
https://www.ncbi.nlm.nih.gov/pubmed/23469167
http://dx.doi.org/10.1371/journal.pone.0055746
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author Ravoori, Murali
Duggal, Jyoti
Gagea, Mihai
Han, Lin
Singh, Sheela
Liu, Ping
Wei, Wei
Ragan, Dustin K.
Bankson, James A.
Ma, Jingfei
Kundra, Vikas
author_facet Ravoori, Murali
Duggal, Jyoti
Gagea, Mihai
Han, Lin
Singh, Sheela
Liu, Ping
Wei, Wei
Ragan, Dustin K.
Bankson, James A.
Ma, Jingfei
Kundra, Vikas
author_sort Ravoori, Murali
collection PubMed
description PURPOSE: Prostate imaging requires optimization in young and old mouse models. We tested which MR sequences and field strengths best depict the prostate gland in young and old mice; and, whether prostate MR signal, size, and architecture change with age. TECHNIQUE: Magnetic resonance imaging (MRI) of the prostate of young (2 months) and old (18 months) male nude mice (n = 6) was performed at 4.7 and 7 T and SCID mice (n = 6) at 7 T field strengths, using T1, fat suppressed T1, DWI, T2, fat suppressed T2, as well as T2-based- and proton density-based Dixon “water only” sequences. Images were ranked for best overall sequence for prostate visualization, prostate delineation, and quality of fat suppression. Prostate volume and signal characteristics were compared and histology was performed. RESULTS: T2-based-Dixon “water only” images ranked best overall for prostate visualization and delineation as well as fat suppression (n = 6, P<0.001) at both 4.7 T and 7 T in nude and 7T in SCID mice. Evaluated in nude mice, T2-based Dixon “water only” had greater prostate CNR and lower fat SNR at 7 T than 4.7 T (P<0.001). Prostate volume was less in older than younger mice (n = 6, P<0.02 nude mice; n = 6, P<0.002 SCID mice). Prostate T2 FSE as well as proton density-based and T2-based-Dixon “water only” signal intensity was higher in younger than older mice (P<0.001 nude mice; P<0.01 SCID mice) both at 4.7 and 7 T. This corresponded to an increase in glandular hyperplasia in older mice by histology (P<0.01, n = 6). CONCLUSION: T2-based Dixon “water only” images best depict the mouse prostate in young and old nude mice at 4.7 and 7 T. The mouse prostate decreases in size with age. The decrease in T2 and T2-based Dixon “water only” signal with age corresponds with glandular hyperplasia. Findings suggest age should be an important determinant when choosing models of prostate biology and disease.
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spelling pubmed-35858792013-03-06 Visualizing the Prostate Gland by MR Imaging in Young and Old Mice Ravoori, Murali Duggal, Jyoti Gagea, Mihai Han, Lin Singh, Sheela Liu, Ping Wei, Wei Ragan, Dustin K. Bankson, James A. Ma, Jingfei Kundra, Vikas PLoS One Research Article PURPOSE: Prostate imaging requires optimization in young and old mouse models. We tested which MR sequences and field strengths best depict the prostate gland in young and old mice; and, whether prostate MR signal, size, and architecture change with age. TECHNIQUE: Magnetic resonance imaging (MRI) of the prostate of young (2 months) and old (18 months) male nude mice (n = 6) was performed at 4.7 and 7 T and SCID mice (n = 6) at 7 T field strengths, using T1, fat suppressed T1, DWI, T2, fat suppressed T2, as well as T2-based- and proton density-based Dixon “water only” sequences. Images were ranked for best overall sequence for prostate visualization, prostate delineation, and quality of fat suppression. Prostate volume and signal characteristics were compared and histology was performed. RESULTS: T2-based-Dixon “water only” images ranked best overall for prostate visualization and delineation as well as fat suppression (n = 6, P<0.001) at both 4.7 T and 7 T in nude and 7T in SCID mice. Evaluated in nude mice, T2-based Dixon “water only” had greater prostate CNR and lower fat SNR at 7 T than 4.7 T (P<0.001). Prostate volume was less in older than younger mice (n = 6, P<0.02 nude mice; n = 6, P<0.002 SCID mice). Prostate T2 FSE as well as proton density-based and T2-based-Dixon “water only” signal intensity was higher in younger than older mice (P<0.001 nude mice; P<0.01 SCID mice) both at 4.7 and 7 T. This corresponded to an increase in glandular hyperplasia in older mice by histology (P<0.01, n = 6). CONCLUSION: T2-based Dixon “water only” images best depict the mouse prostate in young and old nude mice at 4.7 and 7 T. The mouse prostate decreases in size with age. The decrease in T2 and T2-based Dixon “water only” signal with age corresponds with glandular hyperplasia. Findings suggest age should be an important determinant when choosing models of prostate biology and disease. Public Library of Science 2013-03-01 /pmc/articles/PMC3585879/ /pubmed/23469167 http://dx.doi.org/10.1371/journal.pone.0055746 Text en © 2013 Ravoori et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ravoori, Murali
Duggal, Jyoti
Gagea, Mihai
Han, Lin
Singh, Sheela
Liu, Ping
Wei, Wei
Ragan, Dustin K.
Bankson, James A.
Ma, Jingfei
Kundra, Vikas
Visualizing the Prostate Gland by MR Imaging in Young and Old Mice
title Visualizing the Prostate Gland by MR Imaging in Young and Old Mice
title_full Visualizing the Prostate Gland by MR Imaging in Young and Old Mice
title_fullStr Visualizing the Prostate Gland by MR Imaging in Young and Old Mice
title_full_unstemmed Visualizing the Prostate Gland by MR Imaging in Young and Old Mice
title_short Visualizing the Prostate Gland by MR Imaging in Young and Old Mice
title_sort visualizing the prostate gland by mr imaging in young and old mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585879/
https://www.ncbi.nlm.nih.gov/pubmed/23469167
http://dx.doi.org/10.1371/journal.pone.0055746
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