Resazurin-based assay for screening bacteria for radiation sensitivity

We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our co...

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Detalles Bibliográficos
Autores principales: Hudman, Deborah A, Sargentini, Neil J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2013
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586403/
https://www.ncbi.nlm.nih.gov/pubmed/23483236
http://dx.doi.org/10.1186/2193-1801-2-55
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author Hudman, Deborah A
Sargentini, Neil J
author_facet Hudman, Deborah A
Sargentini, Neil J
author_sort Hudman, Deborah A
collection PubMed
description We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours.
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spelling pubmed-35864032013-03-07 Resazurin-based assay for screening bacteria for radiation sensitivity Hudman, Deborah A Sargentini, Neil J Springerplus Methodology We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours. Springer International Publishing AG 2013-02-16 /pmc/articles/PMC3586403/ /pubmed/23483236 http://dx.doi.org/10.1186/2193-1801-2-55 Text en © Hudman and Sargentini; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Hudman, Deborah A
Sargentini, Neil J
Resazurin-based assay for screening bacteria for radiation sensitivity
title Resazurin-based assay for screening bacteria for radiation sensitivity
title_full Resazurin-based assay for screening bacteria for radiation sensitivity
title_fullStr Resazurin-based assay for screening bacteria for radiation sensitivity
title_full_unstemmed Resazurin-based assay for screening bacteria for radiation sensitivity
title_short Resazurin-based assay for screening bacteria for radiation sensitivity
title_sort resazurin-based assay for screening bacteria for radiation sensitivity
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586403/
https://www.ncbi.nlm.nih.gov/pubmed/23483236
http://dx.doi.org/10.1186/2193-1801-2-55
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