Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain

OBJECTIVE(S): In this study we investigated the expression of GABA(A) receptor subunits during brain development. These receptors may change in the embryonic chick forebrain. MATERIALS AND METHODES: The expression levels of four types of GABA(A) receptor gamma subunits (γ1, γ2, γ3 and γ4) were quant...

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Autores principales: Zaker, Sayed Rasul, Esmaeili, Abolghasem, Bouzari, Majid, Shirani, Elham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586925/
https://www.ncbi.nlm.nih.gov/pubmed/23493672
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author Zaker, Sayed Rasul
Esmaeili, Abolghasem
Bouzari, Majid
Shirani, Elham
author_facet Zaker, Sayed Rasul
Esmaeili, Abolghasem
Bouzari, Majid
Shirani, Elham
author_sort Zaker, Sayed Rasul
collection PubMed
description OBJECTIVE(S): In this study we investigated the expression of GABA(A) receptor subunits during brain development. These receptors may change in the embryonic chick forebrain. MATERIALS AND METHODES: The expression levels of four types of GABA(A) receptor gamma subunits (γ1, γ2, γ3 and γ4) were quantified in the embryonic chick forebrain at 32 hr, 3, 7, 14, and 20 days of incubation and day one after hatching. The expression level of mRNA in the forebrain of embryonic chicken was measured using real-time RT-PCR. RESULTS: The expression level of each subunit increased gradually with development and reached a plateau on 20th day of embryonic development. A reduction was observed on day one after hatching in all gamma subunits. CONCLUSION: This may explain the different physiological and pharmacological function of GAB(A) receptor gamma subunits before and after hatching.
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spelling pubmed-35869252013-03-14 Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain Zaker, Sayed Rasul Esmaeili, Abolghasem Bouzari, Majid Shirani, Elham Iran J Basic Med Sci Original Article OBJECTIVE(S): In this study we investigated the expression of GABA(A) receptor subunits during brain development. These receptors may change in the embryonic chick forebrain. MATERIALS AND METHODES: The expression levels of four types of GABA(A) receptor gamma subunits (γ1, γ2, γ3 and γ4) were quantified in the embryonic chick forebrain at 32 hr, 3, 7, 14, and 20 days of incubation and day one after hatching. The expression level of mRNA in the forebrain of embryonic chicken was measured using real-time RT-PCR. RESULTS: The expression level of each subunit increased gradually with development and reached a plateau on 20th day of embryonic development. A reduction was observed on day one after hatching in all gamma subunits. CONCLUSION: This may explain the different physiological and pharmacological function of GAB(A) receptor gamma subunits before and after hatching. Mashhad University of Medical Sciences 2012 /pmc/articles/PMC3586925/ /pubmed/23493672 Text en © 2012: Iranian Journal of Basic Medical Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zaker, Sayed Rasul
Esmaeili, Abolghasem
Bouzari, Majid
Shirani, Elham
Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain
title Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain
title_full Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain
title_fullStr Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain
title_full_unstemmed Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain
title_short Quantitative Analysis of GABA(A) Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain
title_sort quantitative analysis of gaba(a) gamma receptor subunits in the developing embryonic chick forebrain
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586925/
https://www.ncbi.nlm.nih.gov/pubmed/23493672
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