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Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning
The recent literature provides evidence that epigenetic mechanisms such as DNA methylation and histone modification are crucial to gene transcription linked to synaptic plasticity in the mammalian brain - notably in the hippocampus - and memory formation. We measured global histone acetylation level...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587615/ https://www.ncbi.nlm.nih.gov/pubmed/23469244 http://dx.doi.org/10.1371/journal.pone.0057816 |
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author | Bousiges, Olivier Neidl, Romain Majchrzak, Monique Muller, Marc-Antoine Barbelivien, Alexandra Pereira de Vasconcelos, Anne Schneider, Anne Loeffler, Jean-Philippe Cassel, Jean-Christophe Boutillier, Anne-Laurence |
author_facet | Bousiges, Olivier Neidl, Romain Majchrzak, Monique Muller, Marc-Antoine Barbelivien, Alexandra Pereira de Vasconcelos, Anne Schneider, Anne Loeffler, Jean-Philippe Cassel, Jean-Christophe Boutillier, Anne-Laurence |
author_sort | Bousiges, Olivier |
collection | PubMed |
description | The recent literature provides evidence that epigenetic mechanisms such as DNA methylation and histone modification are crucial to gene transcription linked to synaptic plasticity in the mammalian brain - notably in the hippocampus - and memory formation. We measured global histone acetylation levels in the rat hippocampus at an early stage of spatial or fear memory formation. We found that H3, H4 and H2B underwent differential acetylation at specific sites depending on whether rats had been exposed to the context of a task without having to learn or had to learn about a place or fear therein: H3K9K14 acetylation was mostly responsive to any experimental conditions compared to naive animals, whereas H2B N-terminus and H4K12 acetylations were mostly associated with memory for either spatial or fear learning. Altogether, these data suggest that behavior/experience-dependent changes differently regulate specific acetylation modifications of histones in the hippocampus, depending on whether a memory trace is established or not: tagging of H3K9K14 could be associated with perception/processing of testing-related manipulations and context, thereby enhancing chromatin accessibility, while tagging of H2B N-terminus tail and H4K12 could be more closely associated with the formation of memories requiring an engagement of the hippocampus. |
format | Online Article Text |
id | pubmed-3587615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35876152013-03-06 Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning Bousiges, Olivier Neidl, Romain Majchrzak, Monique Muller, Marc-Antoine Barbelivien, Alexandra Pereira de Vasconcelos, Anne Schneider, Anne Loeffler, Jean-Philippe Cassel, Jean-Christophe Boutillier, Anne-Laurence PLoS One Research Article The recent literature provides evidence that epigenetic mechanisms such as DNA methylation and histone modification are crucial to gene transcription linked to synaptic plasticity in the mammalian brain - notably in the hippocampus - and memory formation. We measured global histone acetylation levels in the rat hippocampus at an early stage of spatial or fear memory formation. We found that H3, H4 and H2B underwent differential acetylation at specific sites depending on whether rats had been exposed to the context of a task without having to learn or had to learn about a place or fear therein: H3K9K14 acetylation was mostly responsive to any experimental conditions compared to naive animals, whereas H2B N-terminus and H4K12 acetylations were mostly associated with memory for either spatial or fear learning. Altogether, these data suggest that behavior/experience-dependent changes differently regulate specific acetylation modifications of histones in the hippocampus, depending on whether a memory trace is established or not: tagging of H3K9K14 could be associated with perception/processing of testing-related manipulations and context, thereby enhancing chromatin accessibility, while tagging of H2B N-terminus tail and H4K12 could be more closely associated with the formation of memories requiring an engagement of the hippocampus. Public Library of Science 2013-03-04 /pmc/articles/PMC3587615/ /pubmed/23469244 http://dx.doi.org/10.1371/journal.pone.0057816 Text en © 2013 Bousiges et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bousiges, Olivier Neidl, Romain Majchrzak, Monique Muller, Marc-Antoine Barbelivien, Alexandra Pereira de Vasconcelos, Anne Schneider, Anne Loeffler, Jean-Philippe Cassel, Jean-Christophe Boutillier, Anne-Laurence Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning |
title | Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning |
title_full | Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning |
title_fullStr | Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning |
title_full_unstemmed | Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning |
title_short | Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning |
title_sort | detection of histone acetylation levels in the dorsal hippocampus reveals early tagging on specific residues of h2b and h4 histones in response to learning |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587615/ https://www.ncbi.nlm.nih.gov/pubmed/23469244 http://dx.doi.org/10.1371/journal.pone.0057816 |
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