Cargando…

Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning

The recent literature provides evidence that epigenetic mechanisms such as DNA methylation and histone modification are crucial to gene transcription linked to synaptic plasticity in the mammalian brain - notably in the hippocampus - and memory formation. We measured global histone acetylation level...

Descripción completa

Detalles Bibliográficos
Autores principales: Bousiges, Olivier, Neidl, Romain, Majchrzak, Monique, Muller, Marc-Antoine, Barbelivien, Alexandra, Pereira de Vasconcelos, Anne, Schneider, Anne, Loeffler, Jean-Philippe, Cassel, Jean-Christophe, Boutillier, Anne-Laurence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587615/
https://www.ncbi.nlm.nih.gov/pubmed/23469244
http://dx.doi.org/10.1371/journal.pone.0057816
_version_ 1782261421536444416
author Bousiges, Olivier
Neidl, Romain
Majchrzak, Monique
Muller, Marc-Antoine
Barbelivien, Alexandra
Pereira de Vasconcelos, Anne
Schneider, Anne
Loeffler, Jean-Philippe
Cassel, Jean-Christophe
Boutillier, Anne-Laurence
author_facet Bousiges, Olivier
Neidl, Romain
Majchrzak, Monique
Muller, Marc-Antoine
Barbelivien, Alexandra
Pereira de Vasconcelos, Anne
Schneider, Anne
Loeffler, Jean-Philippe
Cassel, Jean-Christophe
Boutillier, Anne-Laurence
author_sort Bousiges, Olivier
collection PubMed
description The recent literature provides evidence that epigenetic mechanisms such as DNA methylation and histone modification are crucial to gene transcription linked to synaptic plasticity in the mammalian brain - notably in the hippocampus - and memory formation. We measured global histone acetylation levels in the rat hippocampus at an early stage of spatial or fear memory formation. We found that H3, H4 and H2B underwent differential acetylation at specific sites depending on whether rats had been exposed to the context of a task without having to learn or had to learn about a place or fear therein: H3K9K14 acetylation was mostly responsive to any experimental conditions compared to naive animals, whereas H2B N-terminus and H4K12 acetylations were mostly associated with memory for either spatial or fear learning. Altogether, these data suggest that behavior/experience-dependent changes differently regulate specific acetylation modifications of histones in the hippocampus, depending on whether a memory trace is established or not: tagging of H3K9K14 could be associated with perception/processing of testing-related manipulations and context, thereby enhancing chromatin accessibility, while tagging of H2B N-terminus tail and H4K12 could be more closely associated with the formation of memories requiring an engagement of the hippocampus.
format Online
Article
Text
id pubmed-3587615
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35876152013-03-06 Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning Bousiges, Olivier Neidl, Romain Majchrzak, Monique Muller, Marc-Antoine Barbelivien, Alexandra Pereira de Vasconcelos, Anne Schneider, Anne Loeffler, Jean-Philippe Cassel, Jean-Christophe Boutillier, Anne-Laurence PLoS One Research Article The recent literature provides evidence that epigenetic mechanisms such as DNA methylation and histone modification are crucial to gene transcription linked to synaptic plasticity in the mammalian brain - notably in the hippocampus - and memory formation. We measured global histone acetylation levels in the rat hippocampus at an early stage of spatial or fear memory formation. We found that H3, H4 and H2B underwent differential acetylation at specific sites depending on whether rats had been exposed to the context of a task without having to learn or had to learn about a place or fear therein: H3K9K14 acetylation was mostly responsive to any experimental conditions compared to naive animals, whereas H2B N-terminus and H4K12 acetylations were mostly associated with memory for either spatial or fear learning. Altogether, these data suggest that behavior/experience-dependent changes differently regulate specific acetylation modifications of histones in the hippocampus, depending on whether a memory trace is established or not: tagging of H3K9K14 could be associated with perception/processing of testing-related manipulations and context, thereby enhancing chromatin accessibility, while tagging of H2B N-terminus tail and H4K12 could be more closely associated with the formation of memories requiring an engagement of the hippocampus. Public Library of Science 2013-03-04 /pmc/articles/PMC3587615/ /pubmed/23469244 http://dx.doi.org/10.1371/journal.pone.0057816 Text en © 2013 Bousiges et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bousiges, Olivier
Neidl, Romain
Majchrzak, Monique
Muller, Marc-Antoine
Barbelivien, Alexandra
Pereira de Vasconcelos, Anne
Schneider, Anne
Loeffler, Jean-Philippe
Cassel, Jean-Christophe
Boutillier, Anne-Laurence
Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning
title Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning
title_full Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning
title_fullStr Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning
title_full_unstemmed Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning
title_short Detection of Histone Acetylation Levels in the Dorsal Hippocampus Reveals Early Tagging on Specific Residues of H2B and H4 Histones in Response to Learning
title_sort detection of histone acetylation levels in the dorsal hippocampus reveals early tagging on specific residues of h2b and h4 histones in response to learning
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587615/
https://www.ncbi.nlm.nih.gov/pubmed/23469244
http://dx.doi.org/10.1371/journal.pone.0057816
work_keys_str_mv AT bousigesolivier detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT neidlromain detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT majchrzakmonique detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT mullermarcantoine detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT barbelivienalexandra detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT pereiradevasconcelosanne detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT schneideranne detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT loefflerjeanphilippe detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT casseljeanchristophe detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning
AT boutillierannelaurence detectionofhistoneacetylationlevelsinthedorsalhippocampusrevealsearlytaggingonspecificresiduesofh2bandh4histonesinresponsetolearning