Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system....

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Autores principales: Selahi, Fatemeh, Namavari, Mehdi, Hosseini, Mohammad Hossein, Mansourian, Maryam, Tahamtan, Yahya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2013
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587742/
https://www.ncbi.nlm.nih.gov/pubmed/23467930
http://dx.doi.org/10.3347/kjp.2013.51.1.129
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author Selahi, Fatemeh
Namavari, Mehdi
Hosseini, Mohammad Hossein
Mansourian, Maryam
Tahamtan, Yahya
author_facet Selahi, Fatemeh
Namavari, Mehdi
Hosseini, Mohammad Hossein
Mansourian, Maryam
Tahamtan, Yahya
author_sort Selahi, Fatemeh
collection PubMed
description In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.
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spelling pubmed-35877422013-03-06 Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle Selahi, Fatemeh Namavari, Mehdi Hosseini, Mohammad Hossein Mansourian, Maryam Tahamtan, Yahya Korean J Parasitol Brief Communication In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle. The Korean Society for Parasitology and Tropical Medicine 2013-02 2013-02-18 /pmc/articles/PMC3587742/ /pubmed/23467930 http://dx.doi.org/10.3347/kjp.2013.51.1.129 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Selahi, Fatemeh
Namavari, Mehdi
Hosseini, Mohammad Hossein
Mansourian, Maryam
Tahamtan, Yahya
Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
title Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
title_full Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
title_fullStr Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
title_full_unstemmed Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
title_short Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
title_sort development of a disperse dye immunoassay technique for detection of antibodies against neospora caninum in cattle
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587742/
https://www.ncbi.nlm.nih.gov/pubmed/23467930
http://dx.doi.org/10.3347/kjp.2013.51.1.129
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