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Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro

The excitatory action of gamma-aminobutyric acid (GABA) is considered to be a hallmark of the developing nervous system. However, in immature brain slices, excitatory GABA actions may be secondary to neuronal injury during slice preparation. Here, we explored GABA actions in the rodent intact hippoc...

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Autores principales: Valeeva, Guzel, Valiullina, Fliza, Khazipov, Roustem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587803/
https://www.ncbi.nlm.nih.gov/pubmed/23467988
http://dx.doi.org/10.3389/fncel.2013.00020
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author Valeeva, Guzel
Valiullina, Fliza
Khazipov, Roustem
author_facet Valeeva, Guzel
Valiullina, Fliza
Khazipov, Roustem
author_sort Valeeva, Guzel
collection PubMed
description The excitatory action of gamma-aminobutyric acid (GABA) is considered to be a hallmark of the developing nervous system. However, in immature brain slices, excitatory GABA actions may be secondary to neuronal injury during slice preparation. Here, we explored GABA actions in the rodent intact hippocampal preparations and at different depths of hippocampal slices during the early post-natal period [post-natal days (P) 1–7]. We found that in the intact hippocampus at P1–3: (i) GABA exerts depolarizing action as seen in cell-attached single GABA(A) channel recordings; (ii) GABA(A) receptor (GABA(A)-R) agonist isoguvacine and synaptic activation of the GABA(A)-Rs increase the frequency of multiple unit activity and the frequency of the network-driven giant depolarizing potentials (GDPs); and that (iii) Na(+)–K(+)–2Cl(-) cotransporter (NKCC1) antagonist bumetanide suppresses GDPs and the excitatory actions of isoguvacine. In the hippocampal slices at P2–5, isoguvacine and synaptic activation of GABA(A)-Rs-evoked excitatory responses at all slice depths, including surface and core. Thus, GABA exerts excitatory actions in the intact hippocampus (P1–3) and at all depths of hippocampal slices (P2–5). Therefore, the excitatory actions of GABA in hippocampal slices during the first post-natal days are not due to neuronal injury during slice preparation, and the trauma-related excitatory GABA actions at the slice surface are a fundamentally different phenomenon observed during the second post-natal week.
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spelling pubmed-35878032013-03-06 Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro Valeeva, Guzel Valiullina, Fliza Khazipov, Roustem Front Cell Neurosci Neuroscience The excitatory action of gamma-aminobutyric acid (GABA) is considered to be a hallmark of the developing nervous system. However, in immature brain slices, excitatory GABA actions may be secondary to neuronal injury during slice preparation. Here, we explored GABA actions in the rodent intact hippocampal preparations and at different depths of hippocampal slices during the early post-natal period [post-natal days (P) 1–7]. We found that in the intact hippocampus at P1–3: (i) GABA exerts depolarizing action as seen in cell-attached single GABA(A) channel recordings; (ii) GABA(A) receptor (GABA(A)-R) agonist isoguvacine and synaptic activation of the GABA(A)-Rs increase the frequency of multiple unit activity and the frequency of the network-driven giant depolarizing potentials (GDPs); and that (iii) Na(+)–K(+)–2Cl(-) cotransporter (NKCC1) antagonist bumetanide suppresses GDPs and the excitatory actions of isoguvacine. In the hippocampal slices at P2–5, isoguvacine and synaptic activation of GABA(A)-Rs-evoked excitatory responses at all slice depths, including surface and core. Thus, GABA exerts excitatory actions in the intact hippocampus (P1–3) and at all depths of hippocampal slices (P2–5). Therefore, the excitatory actions of GABA in hippocampal slices during the first post-natal days are not due to neuronal injury during slice preparation, and the trauma-related excitatory GABA actions at the slice surface are a fundamentally different phenomenon observed during the second post-natal week. Frontiers Media S.A. 2013-03-05 /pmc/articles/PMC3587803/ /pubmed/23467988 http://dx.doi.org/10.3389/fncel.2013.00020 Text en Copyright © Valeeva, Valiullina and Khazipov. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Neuroscience
Valeeva, Guzel
Valiullina, Fliza
Khazipov, Roustem
Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro
title Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro
title_full Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro
title_fullStr Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro
title_full_unstemmed Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro
title_short Excitatory actions of GABA in the intact neonatal rodent hippocampus in vitro
title_sort excitatory actions of gaba in the intact neonatal rodent hippocampus in vitro
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587803/
https://www.ncbi.nlm.nih.gov/pubmed/23467988
http://dx.doi.org/10.3389/fncel.2013.00020
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