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Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors
Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat’s pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmissi...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587996/ https://www.ncbi.nlm.nih.gov/pubmed/23354478 http://dx.doi.org/10.3390/ijms14022449 |
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author | Katsnelson, Boris A. Privalova, Larisa I. Gurvich, Vladimir B. Makeyev, Oleg H. Shur, Vladimir Ya. Beikin, Yakov B. Sutunkova, Marina P. Kireyeva, Ekaterina P. Minigalieva, Ilzira A. Loginova, Nadezhda V. Vasilyeva, Marina S. Korotkov, Artem V. Shuman, Eugene A. Vlasova, Larisa A. Shishkina, Ekaterina V. Tyurnina, Anastasia E. Kozin, Roman V. Valamina, Irene E. Pichugova, Svetlana V. Tulakina, Ludmila G. |
author_facet | Katsnelson, Boris A. Privalova, Larisa I. Gurvich, Vladimir B. Makeyev, Oleg H. Shur, Vladimir Ya. Beikin, Yakov B. Sutunkova, Marina P. Kireyeva, Ekaterina P. Minigalieva, Ilzira A. Loginova, Nadezhda V. Vasilyeva, Marina S. Korotkov, Artem V. Shuman, Eugene A. Vlasova, Larisa A. Shishkina, Ekaterina V. Tyurnina, Anastasia E. Kozin, Roman V. Valamina, Irene E. Pichugova, Svetlana V. Tulakina, Ludmila G. |
author_sort | Katsnelson, Boris A. |
collection | PubMed |
description | Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat’s pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmission electron, and semi-contact atomic force microscopy. NG and NS were also repeatedly injected intraperitoneally into rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week, up to 20 injections. A group of rats was thus injected with NS after oral administration of a “bioprotective complex” (BPC) comprised of pectin, multivitamins, some amino acids, calcium, selenium, and omega-3 PUFA. After the termination of the injections, many functional and biochemical indices and histopathological features of the spleen, kidneys and liver were evaluated for signs of toxicity, and accumulation of NG or NS in these organs was measured. From the same rats, we obtained cell suspensions of different tissues for performing the RAPD test. It was demonstrated that, although both nanometals were adversely bioactive in all respects considered in this study, NS was more noxious as compared with NG, and that the BPC tested by us attenuated both the toxicity and genotoxicity of NS. |
format | Online Article Text |
id | pubmed-3587996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-35879962013-03-13 Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors Katsnelson, Boris A. Privalova, Larisa I. Gurvich, Vladimir B. Makeyev, Oleg H. Shur, Vladimir Ya. Beikin, Yakov B. Sutunkova, Marina P. Kireyeva, Ekaterina P. Minigalieva, Ilzira A. Loginova, Nadezhda V. Vasilyeva, Marina S. Korotkov, Artem V. Shuman, Eugene A. Vlasova, Larisa A. Shishkina, Ekaterina V. Tyurnina, Anastasia E. Kozin, Roman V. Valamina, Irene E. Pichugova, Svetlana V. Tulakina, Ludmila G. Int J Mol Sci Article Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat’s pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmission electron, and semi-contact atomic force microscopy. NG and NS were also repeatedly injected intraperitoneally into rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week, up to 20 injections. A group of rats was thus injected with NS after oral administration of a “bioprotective complex” (BPC) comprised of pectin, multivitamins, some amino acids, calcium, selenium, and omega-3 PUFA. After the termination of the injections, many functional and biochemical indices and histopathological features of the spleen, kidneys and liver were evaluated for signs of toxicity, and accumulation of NG or NS in these organs was measured. From the same rats, we obtained cell suspensions of different tissues for performing the RAPD test. It was demonstrated that, although both nanometals were adversely bioactive in all respects considered in this study, NS was more noxious as compared with NG, and that the BPC tested by us attenuated both the toxicity and genotoxicity of NS. MDPI 2013-01-25 /pmc/articles/PMC3587996/ /pubmed/23354478 http://dx.doi.org/10.3390/ijms14022449 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Katsnelson, Boris A. Privalova, Larisa I. Gurvich, Vladimir B. Makeyev, Oleg H. Shur, Vladimir Ya. Beikin, Yakov B. Sutunkova, Marina P. Kireyeva, Ekaterina P. Minigalieva, Ilzira A. Loginova, Nadezhda V. Vasilyeva, Marina S. Korotkov, Artem V. Shuman, Eugene A. Vlasova, Larisa A. Shishkina, Ekaterina V. Tyurnina, Anastasia E. Kozin, Roman V. Valamina, Irene E. Pichugova, Svetlana V. Tulakina, Ludmila G. Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors |
title | Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors |
title_full | Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors |
title_fullStr | Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors |
title_full_unstemmed | Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors |
title_short | Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors |
title_sort | comparative in vivo assessment of some adverse bioeffects of equidimensional gold and silver nanoparticles and the attenuation of nanosilver’s effects with a complex of innocuous bioprotectors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587996/ https://www.ncbi.nlm.nih.gov/pubmed/23354478 http://dx.doi.org/10.3390/ijms14022449 |
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