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The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage

Although rapeseed (Brassica napus L.) is known to be affected by waterlogging, the genetic basis of waterlogging tolerance by rapeseed is largely unknown. In this study, the transcriptome under 0 h and 12 h of waterlogging was assayed in the roots of ZS9, a tolerant variety, using digital gene expre...

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Autores principales: Zou, Xiling, Tan, Xiaoyu, Hu, Chengwei, Zeng, Liu, Lu, Guangyuan, Fu, Guiping, Cheng, Yong, Zhang, Xuekun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588007/
https://www.ncbi.nlm.nih.gov/pubmed/23358252
http://dx.doi.org/10.3390/ijms14022637
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author Zou, Xiling
Tan, Xiaoyu
Hu, Chengwei
Zeng, Liu
Lu, Guangyuan
Fu, Guiping
Cheng, Yong
Zhang, Xuekun
author_facet Zou, Xiling
Tan, Xiaoyu
Hu, Chengwei
Zeng, Liu
Lu, Guangyuan
Fu, Guiping
Cheng, Yong
Zhang, Xuekun
author_sort Zou, Xiling
collection PubMed
description Although rapeseed (Brassica napus L.) is known to be affected by waterlogging, the genetic basis of waterlogging tolerance by rapeseed is largely unknown. In this study, the transcriptome under 0 h and 12 h of waterlogging was assayed in the roots of ZS9, a tolerant variety, using digital gene expression (DGE). A total of 4432 differentially expressed genes were identified, indicating that the response to waterlogging in rapeseed is complicated. The assignments of the annotated genes based on GO (Gene Ontology) revealed there were more genes induced under waterlogging in “oxidation reduction”, “secondary metabolism”, “transcription regulation”, and “translation regulation”; suggesting these four pathways are enhanced under waterlogging. Analysis of the 200 most highly expressed genes illustrated that 144 under normal conditions were down-regulated by waterlogging, while up to 191 under waterlogging were those induced in response to stress. The expression of genes involved under waterlogging is mediated by multiple levels of transcriptional, post-transcriptional, translational and post-translational regulation, including phosphorylation and protein degradation; in particular, protein degradation might be involved in the negative regulation in response to this stress. Our results provide new insight into the response to waterlogging and will help to identify important candidate genes.
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spelling pubmed-35880072013-03-13 The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage Zou, Xiling Tan, Xiaoyu Hu, Chengwei Zeng, Liu Lu, Guangyuan Fu, Guiping Cheng, Yong Zhang, Xuekun Int J Mol Sci Article Although rapeseed (Brassica napus L.) is known to be affected by waterlogging, the genetic basis of waterlogging tolerance by rapeseed is largely unknown. In this study, the transcriptome under 0 h and 12 h of waterlogging was assayed in the roots of ZS9, a tolerant variety, using digital gene expression (DGE). A total of 4432 differentially expressed genes were identified, indicating that the response to waterlogging in rapeseed is complicated. The assignments of the annotated genes based on GO (Gene Ontology) revealed there were more genes induced under waterlogging in “oxidation reduction”, “secondary metabolism”, “transcription regulation”, and “translation regulation”; suggesting these four pathways are enhanced under waterlogging. Analysis of the 200 most highly expressed genes illustrated that 144 under normal conditions were down-regulated by waterlogging, while up to 191 under waterlogging were those induced in response to stress. The expression of genes involved under waterlogging is mediated by multiple levels of transcriptional, post-transcriptional, translational and post-translational regulation, including phosphorylation and protein degradation; in particular, protein degradation might be involved in the negative regulation in response to this stress. Our results provide new insight into the response to waterlogging and will help to identify important candidate genes. MDPI 2013-01-28 /pmc/articles/PMC3588007/ /pubmed/23358252 http://dx.doi.org/10.3390/ijms14022637 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zou, Xiling
Tan, Xiaoyu
Hu, Chengwei
Zeng, Liu
Lu, Guangyuan
Fu, Guiping
Cheng, Yong
Zhang, Xuekun
The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage
title The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage
title_full The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage
title_fullStr The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage
title_full_unstemmed The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage
title_short The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage
title_sort transcriptome of brassica napus l. roots under waterlogging at the seedling stage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588007/
https://www.ncbi.nlm.nih.gov/pubmed/23358252
http://dx.doi.org/10.3390/ijms14022637
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