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Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage

Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) commonly coexist in smokers, and the presence of COPD increases the risk of developing LC. Cigarette smoke causes oxidative stress and an inflammatory response in lung cells, which in turn may be involved in COPD and lung cancer devel...

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Autores principales: Pastor, Maria Dolores, Nogal, Ana, Molina-Pinelo, Sonia, Meléndez, Ricardo, Romero-Romero, Beatriz, Mediano, Maria Dolores, López-Campos, Jose L., García-Carbonero, Rocío, Sanchez-Gastaldo, Amparo, Carnero, Amancio, Paz-Ares, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588051/
https://www.ncbi.nlm.nih.gov/pubmed/23389041
http://dx.doi.org/10.3390/ijms14023440
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author Pastor, Maria Dolores
Nogal, Ana
Molina-Pinelo, Sonia
Meléndez, Ricardo
Romero-Romero, Beatriz
Mediano, Maria Dolores
López-Campos, Jose L.
García-Carbonero, Rocío
Sanchez-Gastaldo, Amparo
Carnero, Amancio
Paz-Ares, Luis
author_facet Pastor, Maria Dolores
Nogal, Ana
Molina-Pinelo, Sonia
Meléndez, Ricardo
Romero-Romero, Beatriz
Mediano, Maria Dolores
López-Campos, Jose L.
García-Carbonero, Rocío
Sanchez-Gastaldo, Amparo
Carnero, Amancio
Paz-Ares, Luis
author_sort Pastor, Maria Dolores
collection PubMed
description Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) commonly coexist in smokers, and the presence of COPD increases the risk of developing LC. Cigarette smoke causes oxidative stress and an inflammatory response in lung cells, which in turn may be involved in COPD and lung cancer development. The aim of this study was to identify differential proteomic profiles related to oxidative stress response that were potentially involved in these two pathological entities. Protein content was assessed in the bronchoalveolar lavage (BAL) of 60 patients classified in four groups: COPD, COPD and LC, LC, and control (neither COPD nor LC). Proteins were separated into spots by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and examined by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF). A total of 16 oxidative stress regulatory proteins were differentially expressed in BAL samples from LC and/or COPD patients as compared with the control group. A distinct proteomic reactive oxygen species (ROS) protein signature emerged that characterized lung cancer and COPD. In conclusion, our findings highlight the role of the oxidative stress response proteins in the pathogenic pathways of both diseases, and provide new candidate biomarkers and predictive tools for LC and COPD diagnosis.
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spelling pubmed-35880512013-03-13 Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage Pastor, Maria Dolores Nogal, Ana Molina-Pinelo, Sonia Meléndez, Ricardo Romero-Romero, Beatriz Mediano, Maria Dolores López-Campos, Jose L. García-Carbonero, Rocío Sanchez-Gastaldo, Amparo Carnero, Amancio Paz-Ares, Luis Int J Mol Sci Article Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) commonly coexist in smokers, and the presence of COPD increases the risk of developing LC. Cigarette smoke causes oxidative stress and an inflammatory response in lung cells, which in turn may be involved in COPD and lung cancer development. The aim of this study was to identify differential proteomic profiles related to oxidative stress response that were potentially involved in these two pathological entities. Protein content was assessed in the bronchoalveolar lavage (BAL) of 60 patients classified in four groups: COPD, COPD and LC, LC, and control (neither COPD nor LC). Proteins were separated into spots by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and examined by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF). A total of 16 oxidative stress regulatory proteins were differentially expressed in BAL samples from LC and/or COPD patients as compared with the control group. A distinct proteomic reactive oxygen species (ROS) protein signature emerged that characterized lung cancer and COPD. In conclusion, our findings highlight the role of the oxidative stress response proteins in the pathogenic pathways of both diseases, and provide new candidate biomarkers and predictive tools for LC and COPD diagnosis. MDPI 2013-02-06 /pmc/articles/PMC3588051/ /pubmed/23389041 http://dx.doi.org/10.3390/ijms14023440 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pastor, Maria Dolores
Nogal, Ana
Molina-Pinelo, Sonia
Meléndez, Ricardo
Romero-Romero, Beatriz
Mediano, Maria Dolores
López-Campos, Jose L.
García-Carbonero, Rocío
Sanchez-Gastaldo, Amparo
Carnero, Amancio
Paz-Ares, Luis
Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage
title Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage
title_full Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage
title_fullStr Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage
title_full_unstemmed Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage
title_short Identification of Oxidative Stress Related Proteins as Biomarkers for Lung Cancer and Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage
title_sort identification of oxidative stress related proteins as biomarkers for lung cancer and chronic obstructive pulmonary disease in bronchoalveolar lavage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588051/
https://www.ncbi.nlm.nih.gov/pubmed/23389041
http://dx.doi.org/10.3390/ijms14023440
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