Cargando…
Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plas...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588058/ https://www.ncbi.nlm.nih.gov/pubmed/23434658 http://dx.doi.org/10.3390/ijms14023556 |
_version_ | 1782261489628872704 |
---|---|
author | An, Na Ou, Jiquan Jiang, Daiming Zhang, Liping Liu, Jingru Fu, Kai Dai, Ying Yang, Daichang |
author_facet | An, Na Ou, Jiquan Jiang, Daiming Zhang, Liping Liu, Jingru Fu, Kai Dai, Ying Yang, Daichang |
author_sort | An, Na |
collection | PubMed |
description | Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plasma and low expression levels of recombinant rbFGF in conventional gene expression systems, we explored the production of recombinant rbFGF in rice grains (Oryza sativa bFGF, OsrbFGF). An expression level of up to 185.66 mg/kg in brown rice was obtained. A simple purification protocol was established with final recovery of 4.49% and resulting in a yield of OsrbFGF reaching up to 8.33 mg/kg OsrbFGF. The functional assay of OsrbFGF indicated that the stimulating cell proliferation activity on NIH/3T3 was the same as with commercialized rbFGF. Wound healing in vivo of OsrbFGF is equivalent to commercialized rbFGF. Our results indicate that rice endosperm is capable of expressing small molecular mass proteins, such as bFGF. This again demonstrates that rice endosperm is a promising system to express various biopharmaceutical proteins. |
format | Online Article Text |
id | pubmed-3588058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-35880582013-03-13 Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds An, Na Ou, Jiquan Jiang, Daiming Zhang, Liping Liu, Jingru Fu, Kai Dai, Ying Yang, Daichang Int J Mol Sci Article Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plasma and low expression levels of recombinant rbFGF in conventional gene expression systems, we explored the production of recombinant rbFGF in rice grains (Oryza sativa bFGF, OsrbFGF). An expression level of up to 185.66 mg/kg in brown rice was obtained. A simple purification protocol was established with final recovery of 4.49% and resulting in a yield of OsrbFGF reaching up to 8.33 mg/kg OsrbFGF. The functional assay of OsrbFGF indicated that the stimulating cell proliferation activity on NIH/3T3 was the same as with commercialized rbFGF. Wound healing in vivo of OsrbFGF is equivalent to commercialized rbFGF. Our results indicate that rice endosperm is capable of expressing small molecular mass proteins, such as bFGF. This again demonstrates that rice endosperm is a promising system to express various biopharmaceutical proteins. MDPI 2013-02-07 /pmc/articles/PMC3588058/ /pubmed/23434658 http://dx.doi.org/10.3390/ijms14023556 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article An, Na Ou, Jiquan Jiang, Daiming Zhang, Liping Liu, Jingru Fu, Kai Dai, Ying Yang, Daichang Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds |
title | Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds |
title_full | Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds |
title_fullStr | Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds |
title_full_unstemmed | Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds |
title_short | Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds |
title_sort | expression of a functional recombinant human basic fibroblast growth factor from transgenic rice seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588058/ https://www.ncbi.nlm.nih.gov/pubmed/23434658 http://dx.doi.org/10.3390/ijms14023556 |
work_keys_str_mv | AT anna expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds AT oujiquan expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds AT jiangdaiming expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds AT zhangliping expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds AT liujingru expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds AT fukai expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds AT daiying expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds AT yangdaichang expressionofafunctionalrecombinanthumanbasicfibroblastgrowthfactorfromtransgenicriceseeds |