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Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds

Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plas...

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Autores principales: An, Na, Ou, Jiquan, Jiang, Daiming, Zhang, Liping, Liu, Jingru, Fu, Kai, Dai, Ying, Yang, Daichang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588058/
https://www.ncbi.nlm.nih.gov/pubmed/23434658
http://dx.doi.org/10.3390/ijms14023556
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author An, Na
Ou, Jiquan
Jiang, Daiming
Zhang, Liping
Liu, Jingru
Fu, Kai
Dai, Ying
Yang, Daichang
author_facet An, Na
Ou, Jiquan
Jiang, Daiming
Zhang, Liping
Liu, Jingru
Fu, Kai
Dai, Ying
Yang, Daichang
author_sort An, Na
collection PubMed
description Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plasma and low expression levels of recombinant rbFGF in conventional gene expression systems, we explored the production of recombinant rbFGF in rice grains (Oryza sativa bFGF, OsrbFGF). An expression level of up to 185.66 mg/kg in brown rice was obtained. A simple purification protocol was established with final recovery of 4.49% and resulting in a yield of OsrbFGF reaching up to 8.33 mg/kg OsrbFGF. The functional assay of OsrbFGF indicated that the stimulating cell proliferation activity on NIH/3T3 was the same as with commercialized rbFGF. Wound healing in vivo of OsrbFGF is equivalent to commercialized rbFGF. Our results indicate that rice endosperm is capable of expressing small molecular mass proteins, such as bFGF. This again demonstrates that rice endosperm is a promising system to express various biopharmaceutical proteins.
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spelling pubmed-35880582013-03-13 Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds An, Na Ou, Jiquan Jiang, Daiming Zhang, Liping Liu, Jingru Fu, Kai Dai, Ying Yang, Daichang Int J Mol Sci Article Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plasma and low expression levels of recombinant rbFGF in conventional gene expression systems, we explored the production of recombinant rbFGF in rice grains (Oryza sativa bFGF, OsrbFGF). An expression level of up to 185.66 mg/kg in brown rice was obtained. A simple purification protocol was established with final recovery of 4.49% and resulting in a yield of OsrbFGF reaching up to 8.33 mg/kg OsrbFGF. The functional assay of OsrbFGF indicated that the stimulating cell proliferation activity on NIH/3T3 was the same as with commercialized rbFGF. Wound healing in vivo of OsrbFGF is equivalent to commercialized rbFGF. Our results indicate that rice endosperm is capable of expressing small molecular mass proteins, such as bFGF. This again demonstrates that rice endosperm is a promising system to express various biopharmaceutical proteins. MDPI 2013-02-07 /pmc/articles/PMC3588058/ /pubmed/23434658 http://dx.doi.org/10.3390/ijms14023556 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
An, Na
Ou, Jiquan
Jiang, Daiming
Zhang, Liping
Liu, Jingru
Fu, Kai
Dai, Ying
Yang, Daichang
Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
title Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
title_full Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
title_fullStr Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
title_full_unstemmed Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
title_short Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
title_sort expression of a functional recombinant human basic fibroblast growth factor from transgenic rice seeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588058/
https://www.ncbi.nlm.nih.gov/pubmed/23434658
http://dx.doi.org/10.3390/ijms14023556
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