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First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems

Miniaturization and parallelization of cell culture procedures are in focus of research in order to develop test platforms with low material consumption and increased standardization for toxicity and drug screenings. The cultivation in hanging drops (HDs) is a convenient and versatile tool for biolo...

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Autores principales: Schulz, Julia C, Stumpf, Patrick S, Katsen-Globa, Alisa, Sachinidis, Agapios, Hescheler, Jürgen, Zimmermann, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588398/
https://www.ncbi.nlm.nih.gov/pubmed/23486530
http://dx.doi.org/10.1002/elsc.201100213
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author Schulz, Julia C
Stumpf, Patrick S
Katsen-Globa, Alisa
Sachinidis, Agapios
Hescheler, Jürgen
Zimmermann, Heiko
author_facet Schulz, Julia C
Stumpf, Patrick S
Katsen-Globa, Alisa
Sachinidis, Agapios
Hescheler, Jürgen
Zimmermann, Heiko
author_sort Schulz, Julia C
collection PubMed
description Miniaturization and parallelization of cell culture procedures are in focus of research in order to develop test platforms with low material consumption and increased standardization for toxicity and drug screenings. The cultivation in hanging drops (HDs) is a convenient and versatile tool for biological applications and represents an interesting model system for the screening applications due to its uniform shape, the advantageous gas supply, and the small volume. However, its application has so far been limited to non‐adherent and aggregate forming cells. Here, we describe for the first time the proof-of-principle regarding the adherent cultivation of human embryonic stem cells in HD. For this microcarriers were added to the droplet as dynamic cultivation surfaces resulting in a maintained pluripotency and proliferation capacity for 10 days. This enables the HD technique to be extended to the cultivation of adherence-dependent stem cells. Also, the possible automation of this method by implementation of liquid handling systems opens new possibilities for miniaturized screenings, the improvement of cultivation and differentiation conditions, and toxicity and drug development.
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spelling pubmed-35883982013-03-11 First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems Schulz, Julia C Stumpf, Patrick S Katsen-Globa, Alisa Sachinidis, Agapios Hescheler, Jürgen Zimmermann, Heiko Eng Life Sci Short Communication Miniaturization and parallelization of cell culture procedures are in focus of research in order to develop test platforms with low material consumption and increased standardization for toxicity and drug screenings. The cultivation in hanging drops (HDs) is a convenient and versatile tool for biological applications and represents an interesting model system for the screening applications due to its uniform shape, the advantageous gas supply, and the small volume. However, its application has so far been limited to non‐adherent and aggregate forming cells. Here, we describe for the first time the proof-of-principle regarding the adherent cultivation of human embryonic stem cells in HD. For this microcarriers were added to the droplet as dynamic cultivation surfaces resulting in a maintained pluripotency and proliferation capacity for 10 days. This enables the HD technique to be extended to the cultivation of adherence-dependent stem cells. Also, the possible automation of this method by implementation of liquid handling systems opens new possibilities for miniaturized screenings, the improvement of cultivation and differentiation conditions, and toxicity and drug development. WILEY-VCH Verlag 2012-11 2012-08-01 /pmc/articles/PMC3588398/ /pubmed/23486530 http://dx.doi.org/10.1002/elsc.201100213 Text en © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Short Communication
Schulz, Julia C
Stumpf, Patrick S
Katsen-Globa, Alisa
Sachinidis, Agapios
Hescheler, Jürgen
Zimmermann, Heiko
First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems
title First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems
title_full First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems
title_fullStr First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems
title_full_unstemmed First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems
title_short First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems
title_sort first steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588398/
https://www.ncbi.nlm.nih.gov/pubmed/23486530
http://dx.doi.org/10.1002/elsc.201100213
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