Cargando…
Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction
In this study, the transcriptome of the Gerbera hybrida ray floret was constructed using a high-throughput Illumina sequencing platform. All 47,104 UniGenes with an average length of 845 nt and an N50 equaling 1321 nt were generated from 72,688,546 total primary reads after filtering and assembly. A...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589416/ https://www.ncbi.nlm.nih.gov/pubmed/23472101 http://dx.doi.org/10.1371/journal.pone.0057715 |
_version_ | 1782261729181302784 |
---|---|
author | Kuang, Qi Li, Lingfei Peng, Jianzong Sun, Shulan Wang, Xiaojing |
author_facet | Kuang, Qi Li, Lingfei Peng, Jianzong Sun, Shulan Wang, Xiaojing |
author_sort | Kuang, Qi |
collection | PubMed |
description | In this study, the transcriptome of the Gerbera hybrida ray floret was constructed using a high-throughput Illumina sequencing platform. All 47,104 UniGenes with an average length of 845 nt and an N50 equaling 1321 nt were generated from 72,688,546 total primary reads after filtering and assembly. A total of 36,693 transcripts were annotated by comparison with non-redundant National Center for Biotechnology Information (NCBI) protein (Nr), non-redundant NCBI nucleotide (Nt), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases after removing exogenous contaminated sequences. The majority of the genes that are associated with gibberellin metabolism and signal transduction were identified. The targets for signal transduction of other plant hormones were also enumerated. Our study provides a systematic overview of the hormone signal transduction genes that are involved in ray floral development in Asteraceae and should facilitate further understanding of the crucial roles of phytohormones in plant growth. |
format | Online Article Text |
id | pubmed-3589416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35894162013-03-07 Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction Kuang, Qi Li, Lingfei Peng, Jianzong Sun, Shulan Wang, Xiaojing PLoS One Research Article In this study, the transcriptome of the Gerbera hybrida ray floret was constructed using a high-throughput Illumina sequencing platform. All 47,104 UniGenes with an average length of 845 nt and an N50 equaling 1321 nt were generated from 72,688,546 total primary reads after filtering and assembly. A total of 36,693 transcripts were annotated by comparison with non-redundant National Center for Biotechnology Information (NCBI) protein (Nr), non-redundant NCBI nucleotide (Nt), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases after removing exogenous contaminated sequences. The majority of the genes that are associated with gibberellin metabolism and signal transduction were identified. The targets for signal transduction of other plant hormones were also enumerated. Our study provides a systematic overview of the hormone signal transduction genes that are involved in ray floral development in Asteraceae and should facilitate further understanding of the crucial roles of phytohormones in plant growth. Public Library of Science 2013-03-05 /pmc/articles/PMC3589416/ /pubmed/23472101 http://dx.doi.org/10.1371/journal.pone.0057715 Text en © 2013 Kuang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kuang, Qi Li, Lingfei Peng, Jianzong Sun, Shulan Wang, Xiaojing Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction |
title | Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction |
title_full | Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction |
title_fullStr | Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction |
title_full_unstemmed | Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction |
title_short | Transcriptome Analysis of Gerbera hybrida Ray Florets: Putative Genes Associated with Gibberellin Metabolism and Signal Transduction |
title_sort | transcriptome analysis of gerbera hybrida ray florets: putative genes associated with gibberellin metabolism and signal transduction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589416/ https://www.ncbi.nlm.nih.gov/pubmed/23472101 http://dx.doi.org/10.1371/journal.pone.0057715 |
work_keys_str_mv | AT kuangqi transcriptomeanalysisofgerberahybridarayfloretsputativegenesassociatedwithgibberellinmetabolismandsignaltransduction AT lilingfei transcriptomeanalysisofgerberahybridarayfloretsputativegenesassociatedwithgibberellinmetabolismandsignaltransduction AT pengjianzong transcriptomeanalysisofgerberahybridarayfloretsputativegenesassociatedwithgibberellinmetabolismandsignaltransduction AT sunshulan transcriptomeanalysisofgerberahybridarayfloretsputativegenesassociatedwithgibberellinmetabolismandsignaltransduction AT wangxiaojing transcriptomeanalysisofgerberahybridarayfloretsputativegenesassociatedwithgibberellinmetabolismandsignaltransduction |