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Malate and Fumarate Extend Lifespan in Caenorhabditis elegans

Malate, the tricarboxylic acid (TCA) cycle metabolite, increased lifespan and thermotolerance in the nematode C. elegans. Malate can be synthesized from fumarate by the enzyme fumarase and further oxidized to oxaloacetate by malate dehydrogenase with the accompanying reduction of NAD. Addition of fu...

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Autores principales: Edwards, Clare B., Copes, Neil, Brito, Andres G., Canfield, John, Bradshaw, Patrick C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589421/
https://www.ncbi.nlm.nih.gov/pubmed/23472183
http://dx.doi.org/10.1371/journal.pone.0058345
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author Edwards, Clare B.
Copes, Neil
Brito, Andres G.
Canfield, John
Bradshaw, Patrick C.
author_facet Edwards, Clare B.
Copes, Neil
Brito, Andres G.
Canfield, John
Bradshaw, Patrick C.
author_sort Edwards, Clare B.
collection PubMed
description Malate, the tricarboxylic acid (TCA) cycle metabolite, increased lifespan and thermotolerance in the nematode C. elegans. Malate can be synthesized from fumarate by the enzyme fumarase and further oxidized to oxaloacetate by malate dehydrogenase with the accompanying reduction of NAD. Addition of fumarate also extended lifespan, but succinate addition did not, although all three intermediates activated nuclear translocation of the cytoprotective DAF-16/FOXO transcription factor and protected from paraquat-induced oxidative stress. The glyoxylate shunt, an anabolic pathway linked to lifespan extension in C. elegans, reversibly converts isocitrate and acetyl-CoA to succinate, malate, and CoA. The increased longevity provided by malate addition did not occur in fumarase (fum-1), glyoxylate shunt (gei-7), succinate dehydrogenase flavoprotein (sdha-2), or soluble fumarate reductase F48E8.3 RNAi knockdown worms. Therefore, to increase lifespan, malate must be first converted to fumarate, then fumarate must be reduced to succinate by soluble fumarate reductase and the mitochondrial electron transport chain complex II. Reduction of fumarate to succinate is coupled with the oxidation of FADH(2) to FAD. Lifespan extension induced by malate depended upon the longevity regulators DAF-16 and SIR-2.1. Malate supplementation did not extend the lifespan of long-lived eat-2 mutant worms, a model of dietary restriction. Malate and fumarate addition increased oxygen consumption, but decreased ATP levels and mitochondrial membrane potential suggesting a mild uncoupling of oxidative phosphorylation. Malate also increased NADPH, NAD, and the NAD/NADH ratio. Fumarate reduction, glyoxylate shunt activity, and mild mitochondrial uncoupling likely contribute to the lifespan extension induced by malate and fumarate by increasing the amount of oxidized NAD and FAD cofactors.
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spelling pubmed-35894212013-03-07 Malate and Fumarate Extend Lifespan in Caenorhabditis elegans Edwards, Clare B. Copes, Neil Brito, Andres G. Canfield, John Bradshaw, Patrick C. PLoS One Research Article Malate, the tricarboxylic acid (TCA) cycle metabolite, increased lifespan and thermotolerance in the nematode C. elegans. Malate can be synthesized from fumarate by the enzyme fumarase and further oxidized to oxaloacetate by malate dehydrogenase with the accompanying reduction of NAD. Addition of fumarate also extended lifespan, but succinate addition did not, although all three intermediates activated nuclear translocation of the cytoprotective DAF-16/FOXO transcription factor and protected from paraquat-induced oxidative stress. The glyoxylate shunt, an anabolic pathway linked to lifespan extension in C. elegans, reversibly converts isocitrate and acetyl-CoA to succinate, malate, and CoA. The increased longevity provided by malate addition did not occur in fumarase (fum-1), glyoxylate shunt (gei-7), succinate dehydrogenase flavoprotein (sdha-2), or soluble fumarate reductase F48E8.3 RNAi knockdown worms. Therefore, to increase lifespan, malate must be first converted to fumarate, then fumarate must be reduced to succinate by soluble fumarate reductase and the mitochondrial electron transport chain complex II. Reduction of fumarate to succinate is coupled with the oxidation of FADH(2) to FAD. Lifespan extension induced by malate depended upon the longevity regulators DAF-16 and SIR-2.1. Malate supplementation did not extend the lifespan of long-lived eat-2 mutant worms, a model of dietary restriction. Malate and fumarate addition increased oxygen consumption, but decreased ATP levels and mitochondrial membrane potential suggesting a mild uncoupling of oxidative phosphorylation. Malate also increased NADPH, NAD, and the NAD/NADH ratio. Fumarate reduction, glyoxylate shunt activity, and mild mitochondrial uncoupling likely contribute to the lifespan extension induced by malate and fumarate by increasing the amount of oxidized NAD and FAD cofactors. Public Library of Science 2013-03-05 /pmc/articles/PMC3589421/ /pubmed/23472183 http://dx.doi.org/10.1371/journal.pone.0058345 Text en © 2013 Edwards et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Edwards, Clare B.
Copes, Neil
Brito, Andres G.
Canfield, John
Bradshaw, Patrick C.
Malate and Fumarate Extend Lifespan in Caenorhabditis elegans
title Malate and Fumarate Extend Lifespan in Caenorhabditis elegans
title_full Malate and Fumarate Extend Lifespan in Caenorhabditis elegans
title_fullStr Malate and Fumarate Extend Lifespan in Caenorhabditis elegans
title_full_unstemmed Malate and Fumarate Extend Lifespan in Caenorhabditis elegans
title_short Malate and Fumarate Extend Lifespan in Caenorhabditis elegans
title_sort malate and fumarate extend lifespan in caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589421/
https://www.ncbi.nlm.nih.gov/pubmed/23472183
http://dx.doi.org/10.1371/journal.pone.0058345
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