Cargando…
HPV Testing by cobas HPV Test in a Population from Catalonia
BACKGROUND: HPV testing in cervical cancer screening has been proposed as an alternative or complementary to cytology in women older than 30 years. However, adequate clinical sensitivity and specificity are crucial for a new test to be implemented. Hybrid Capture 2 (HC2) has proved good clinical per...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590124/ https://www.ncbi.nlm.nih.gov/pubmed/23483984 http://dx.doi.org/10.1371/journal.pone.0058153 |
_version_ | 1782261810054823936 |
---|---|
author | Lloveras, Belén Gomez, Silvia Alameda, Francesc Bellosillo, Beatriz Mojal, Sergi Muset, Mercè Parra, Manuel Palomares, José Carlos Serrano, Sergi |
author_facet | Lloveras, Belén Gomez, Silvia Alameda, Francesc Bellosillo, Beatriz Mojal, Sergi Muset, Mercè Parra, Manuel Palomares, José Carlos Serrano, Sergi |
author_sort | Lloveras, Belén |
collection | PubMed |
description | BACKGROUND: HPV testing in cervical cancer screening has been proposed as an alternative or complementary to cytology in women older than 30 years. However, adequate clinical sensitivity and specificity are crucial for a new test to be implemented. Hybrid Capture 2 (HC2) has proved good clinical performance in selecting women at risk for high-grade intraepithelial lesions with a high sensitivity and specificity. cobas HPV Test has been recently launched and its performance in different clinical settings needs to be determined. OBJECTIVES: The aim of this study was to evaluate the cobas HPV Test for the detection of cervical HPV infection in a population of women in Catalonia (Spain) using HC2 as a reference. MATERIALS AND METHODS: Cervical liquid cytology samples from 958 women have been studied. Sensitivity was analyzed in 60 samples from patients with a high-grade intraepithelial lesion (≥CIN2) on histology and specificity was determined in 898 samples from women with no ≥CIN2. All cases had HC2 and cobas HPV Test performed. Statistical analyses of sensitivity, specificity and comparison between HC2 and cobas HPV Test by a non-inferiority test were applied. RESULTS: Sensitivity of HC2 and cobas HPV Test for detecting ≥CIN2 proved identical (98.3%) while specificity was 85.3% and 86.2% respectively. The non-inferiority test demonstrated that cobas HPV Test surpassed 90% sensitivity and 98% specificity of HC2. CONCLUSION: The cobas HPV Test results fulfilled sensitivity and specificity requirements for HPV based cervical cancer screening and for the triage of minor cytological abnormalities, allowing its introduction in clinical settings. |
format | Online Article Text |
id | pubmed-3590124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35901242013-03-12 HPV Testing by cobas HPV Test in a Population from Catalonia Lloveras, Belén Gomez, Silvia Alameda, Francesc Bellosillo, Beatriz Mojal, Sergi Muset, Mercè Parra, Manuel Palomares, José Carlos Serrano, Sergi PLoS One Research Article BACKGROUND: HPV testing in cervical cancer screening has been proposed as an alternative or complementary to cytology in women older than 30 years. However, adequate clinical sensitivity and specificity are crucial for a new test to be implemented. Hybrid Capture 2 (HC2) has proved good clinical performance in selecting women at risk for high-grade intraepithelial lesions with a high sensitivity and specificity. cobas HPV Test has been recently launched and its performance in different clinical settings needs to be determined. OBJECTIVES: The aim of this study was to evaluate the cobas HPV Test for the detection of cervical HPV infection in a population of women in Catalonia (Spain) using HC2 as a reference. MATERIALS AND METHODS: Cervical liquid cytology samples from 958 women have been studied. Sensitivity was analyzed in 60 samples from patients with a high-grade intraepithelial lesion (≥CIN2) on histology and specificity was determined in 898 samples from women with no ≥CIN2. All cases had HC2 and cobas HPV Test performed. Statistical analyses of sensitivity, specificity and comparison between HC2 and cobas HPV Test by a non-inferiority test were applied. RESULTS: Sensitivity of HC2 and cobas HPV Test for detecting ≥CIN2 proved identical (98.3%) while specificity was 85.3% and 86.2% respectively. The non-inferiority test demonstrated that cobas HPV Test surpassed 90% sensitivity and 98% specificity of HC2. CONCLUSION: The cobas HPV Test results fulfilled sensitivity and specificity requirements for HPV based cervical cancer screening and for the triage of minor cytological abnormalities, allowing its introduction in clinical settings. Public Library of Science 2013-03-06 /pmc/articles/PMC3590124/ /pubmed/23483984 http://dx.doi.org/10.1371/journal.pone.0058153 Text en © 2013 Lloveras et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lloveras, Belén Gomez, Silvia Alameda, Francesc Bellosillo, Beatriz Mojal, Sergi Muset, Mercè Parra, Manuel Palomares, José Carlos Serrano, Sergi HPV Testing by cobas HPV Test in a Population from Catalonia |
title | HPV Testing by cobas HPV Test in a Population from Catalonia |
title_full | HPV Testing by cobas HPV Test in a Population from Catalonia |
title_fullStr | HPV Testing by cobas HPV Test in a Population from Catalonia |
title_full_unstemmed | HPV Testing by cobas HPV Test in a Population from Catalonia |
title_short | HPV Testing by cobas HPV Test in a Population from Catalonia |
title_sort | hpv testing by cobas hpv test in a population from catalonia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590124/ https://www.ncbi.nlm.nih.gov/pubmed/23483984 http://dx.doi.org/10.1371/journal.pone.0058153 |
work_keys_str_mv | AT lloverasbelen hpvtestingbycobashpvtestinapopulationfromcatalonia AT gomezsilvia hpvtestingbycobashpvtestinapopulationfromcatalonia AT alamedafrancesc hpvtestingbycobashpvtestinapopulationfromcatalonia AT bellosillobeatriz hpvtestingbycobashpvtestinapopulationfromcatalonia AT mojalsergi hpvtestingbycobashpvtestinapopulationfromcatalonia AT musetmerce hpvtestingbycobashpvtestinapopulationfromcatalonia AT parramanuel hpvtestingbycobashpvtestinapopulationfromcatalonia AT palomaresjosecarlos hpvtestingbycobashpvtestinapopulationfromcatalonia AT serranosergi hpvtestingbycobashpvtestinapopulationfromcatalonia |