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Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants

The effects of interactions between genetic materials and polycyclic aromatic hydrocarbons (PAHs) on gene expression in the extracellular environment remain to be elucidated and little information is currently available on the effect of ionic strength on the transformation of plasmid DNA exposed to...

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Autores principales: Kang, Fuxing, Wang, Hong, Gao, Yanzheng, Long, Jian, Wang, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590140/
https://www.ncbi.nlm.nih.gov/pubmed/23484001
http://dx.doi.org/10.1371/journal.pone.0058238
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author Kang, Fuxing
Wang, Hong
Gao, Yanzheng
Long, Jian
Wang, Qian
author_facet Kang, Fuxing
Wang, Hong
Gao, Yanzheng
Long, Jian
Wang, Qian
author_sort Kang, Fuxing
collection PubMed
description The effects of interactions between genetic materials and polycyclic aromatic hydrocarbons (PAHs) on gene expression in the extracellular environment remain to be elucidated and little information is currently available on the effect of ionic strength on the transformation of plasmid DNA exposed to PAHs. Phenanthrene and pyrene were used as representative PAHs to evaluate the transformation of plasmid DNA after PAH exposure and to determine the role of Ca(2+) during the transformation. Plasmid DNA exposed to the test PAHs demonstrated low transformation efficiency. In the absence of PAHs, the transformation efficiency was 4.7 log units; however, the efficiency decreased to 3.72–3.14 log units with phenanthrene/pyrene exposures of 50 µg·L(–1). The addition of Ca(2+) enhanced the low transformation efficiency of DNA exposed to PAHs. Based on the co-sorption of Ca(2+) and phenanthrene/pyrene by DNA, we employed Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and mass spectrometry (MS) to determine the mechanisms involved in PAH-induced DNA transformation. The observed low transformation efficiency of DNA exposed to either phenanthrene or pyrene can be attributed to a broken hydrogen bond in the double helix caused by planar PAHs. Added Ca(2+) formed strong electrovalent bonds with “–POO(–)–” groups in the DNA, weakening the interaction between PAHs and DNA based on weak molecular forces. This decreased the damage of PAHs to hydrogen bonds in double-stranded DNA by isolating DNA molecules from PAHs and consequently enhanced the transformation efficiency of DNA exposed to PAH contaminants. The findings provide insight into the effects of anthropogenic trace PAHs on DNA transfer in natural environments.
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spelling pubmed-35901402013-03-12 Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants Kang, Fuxing Wang, Hong Gao, Yanzheng Long, Jian Wang, Qian PLoS One Research Article The effects of interactions between genetic materials and polycyclic aromatic hydrocarbons (PAHs) on gene expression in the extracellular environment remain to be elucidated and little information is currently available on the effect of ionic strength on the transformation of plasmid DNA exposed to PAHs. Phenanthrene and pyrene were used as representative PAHs to evaluate the transformation of plasmid DNA after PAH exposure and to determine the role of Ca(2+) during the transformation. Plasmid DNA exposed to the test PAHs demonstrated low transformation efficiency. In the absence of PAHs, the transformation efficiency was 4.7 log units; however, the efficiency decreased to 3.72–3.14 log units with phenanthrene/pyrene exposures of 50 µg·L(–1). The addition of Ca(2+) enhanced the low transformation efficiency of DNA exposed to PAHs. Based on the co-sorption of Ca(2+) and phenanthrene/pyrene by DNA, we employed Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and mass spectrometry (MS) to determine the mechanisms involved in PAH-induced DNA transformation. The observed low transformation efficiency of DNA exposed to either phenanthrene or pyrene can be attributed to a broken hydrogen bond in the double helix caused by planar PAHs. Added Ca(2+) formed strong electrovalent bonds with “–POO(–)–” groups in the DNA, weakening the interaction between PAHs and DNA based on weak molecular forces. This decreased the damage of PAHs to hydrogen bonds in double-stranded DNA by isolating DNA molecules from PAHs and consequently enhanced the transformation efficiency of DNA exposed to PAH contaminants. The findings provide insight into the effects of anthropogenic trace PAHs on DNA transfer in natural environments. Public Library of Science 2013-03-06 /pmc/articles/PMC3590140/ /pubmed/23484001 http://dx.doi.org/10.1371/journal.pone.0058238 Text en © 2013 Kang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kang, Fuxing
Wang, Hong
Gao, Yanzheng
Long, Jian
Wang, Qian
Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants
title Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants
title_full Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants
title_fullStr Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants
title_full_unstemmed Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants
title_short Ca(2+) Promoted the Low Transformation Efficiency of Plasmid DNA Exposed to PAH Contaminants
title_sort ca(2+) promoted the low transformation efficiency of plasmid dna exposed to pah contaminants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590140/
https://www.ncbi.nlm.nih.gov/pubmed/23484001
http://dx.doi.org/10.1371/journal.pone.0058238
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