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Toward a molecular understanding of RNA remodeling by DEAD-box proteins

DEAD-box proteins are superfamily 2 helicases that function in all aspects of RNA metabolism. They employ ATP binding and hydrolysis to generate tight, yet regulated RNA binding, which is used to unwind short RNA helices non-processively and promote structural transitions of RNA and RNA-protein subs...

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Detalles Bibliográficos
Autores principales: Russell, Rick, Jarmoskaite, Inga, Lambowitz, Alan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590237/
https://www.ncbi.nlm.nih.gov/pubmed/22995827
http://dx.doi.org/10.4161/rna.22210
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author Russell, Rick
Jarmoskaite, Inga
Lambowitz, Alan M.
author_facet Russell, Rick
Jarmoskaite, Inga
Lambowitz, Alan M.
author_sort Russell, Rick
collection PubMed
description DEAD-box proteins are superfamily 2 helicases that function in all aspects of RNA metabolism. They employ ATP binding and hydrolysis to generate tight, yet regulated RNA binding, which is used to unwind short RNA helices non-processively and promote structural transitions of RNA and RNA-protein substrates. In the last few years, substantial progress has been made toward a detailed, quantitative understanding of the structural and biochemical properties of DEAD-box proteins. Concurrently, progress has been made toward a physical understanding of the RNA rearrangements and folding steps that are accelerated by DEAD-box proteins in model systems. Here, we review the recent progress on both of these fronts, focusing on the mitochondrial DEAD-box proteins Mss116 and CYT-19 and their mechanisms in promoting the splicing of group I and group II introns.
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spelling pubmed-35902372013-04-09 Toward a molecular understanding of RNA remodeling by DEAD-box proteins Russell, Rick Jarmoskaite, Inga Lambowitz, Alan M. RNA Biol Review DEAD-box proteins are superfamily 2 helicases that function in all aspects of RNA metabolism. They employ ATP binding and hydrolysis to generate tight, yet regulated RNA binding, which is used to unwind short RNA helices non-processively and promote structural transitions of RNA and RNA-protein substrates. In the last few years, substantial progress has been made toward a detailed, quantitative understanding of the structural and biochemical properties of DEAD-box proteins. Concurrently, progress has been made toward a physical understanding of the RNA rearrangements and folding steps that are accelerated by DEAD-box proteins in model systems. Here, we review the recent progress on both of these fronts, focusing on the mitochondrial DEAD-box proteins Mss116 and CYT-19 and their mechanisms in promoting the splicing of group I and group II introns. Landes Bioscience 2013-01-01 /pmc/articles/PMC3590237/ /pubmed/22995827 http://dx.doi.org/10.4161/rna.22210 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Review
Russell, Rick
Jarmoskaite, Inga
Lambowitz, Alan M.
Toward a molecular understanding of RNA remodeling by DEAD-box proteins
title Toward a molecular understanding of RNA remodeling by DEAD-box proteins
title_full Toward a molecular understanding of RNA remodeling by DEAD-box proteins
title_fullStr Toward a molecular understanding of RNA remodeling by DEAD-box proteins
title_full_unstemmed Toward a molecular understanding of RNA remodeling by DEAD-box proteins
title_short Toward a molecular understanding of RNA remodeling by DEAD-box proteins
title_sort toward a molecular understanding of rna remodeling by dead-box proteins
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590237/
https://www.ncbi.nlm.nih.gov/pubmed/22995827
http://dx.doi.org/10.4161/rna.22210
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