Cargando…
Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis
High temperature impedes the growth and productivity of various crop species. To date, rice (Oryza sativa L.) has not been exploited to understand the molecular basis of its abnormally high level of temperature tolerance. To identify transcripts induced by heat stress, twenty-day-old rice seedlings...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590577/ https://www.ncbi.nlm.nih.gov/pubmed/23509744 http://dx.doi.org/10.1155/2013/576189 |
_version_ | 1782261887453364224 |
---|---|
author | Cao, Yunying Zhang, Qian Chen, Yanhong Zhao, Hua Lang, Youzhong Yu, Chunmei Yang, Jianchang |
author_facet | Cao, Yunying Zhang, Qian Chen, Yanhong Zhao, Hua Lang, Youzhong Yu, Chunmei Yang, Jianchang |
author_sort | Cao, Yunying |
collection | PubMed |
description | High temperature impedes the growth and productivity of various crop species. To date, rice (Oryza sativa L.) has not been exploited to understand the molecular basis of its abnormally high level of temperature tolerance. To identify transcripts induced by heat stress, twenty-day-old rice seedlings of different rice cultivars suffering from heat stress were treated at different times, and differential gene expression analyses in leaves were performed by cDNA-AFLP and further verified by real-time RT-PCR. In aggregate, more than three thousand different fragments were indentified, and 49 fragments were selected for the sequence and differential expressed genes were classified functionally into different groups. 6 of 49 fragments were measured by real-time RT-PCR. In addition, the variations of three different polyamine contents in response to heat stress through high-performance liquid chromatography (HPLC) analysis were also performed. The results and their direct and indirect relationships to heat stress tolerance mechanism were discussed. |
format | Online Article Text |
id | pubmed-3590577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35905772013-03-18 Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis Cao, Yunying Zhang, Qian Chen, Yanhong Zhao, Hua Lang, Youzhong Yu, Chunmei Yang, Jianchang Biomed Res Int Research Article High temperature impedes the growth and productivity of various crop species. To date, rice (Oryza sativa L.) has not been exploited to understand the molecular basis of its abnormally high level of temperature tolerance. To identify transcripts induced by heat stress, twenty-day-old rice seedlings of different rice cultivars suffering from heat stress were treated at different times, and differential gene expression analyses in leaves were performed by cDNA-AFLP and further verified by real-time RT-PCR. In aggregate, more than three thousand different fragments were indentified, and 49 fragments were selected for the sequence and differential expressed genes were classified functionally into different groups. 6 of 49 fragments were measured by real-time RT-PCR. In addition, the variations of three different polyamine contents in response to heat stress through high-performance liquid chromatography (HPLC) analysis were also performed. The results and their direct and indirect relationships to heat stress tolerance mechanism were discussed. Hindawi Publishing Corporation 2013 2013-02-17 /pmc/articles/PMC3590577/ /pubmed/23509744 http://dx.doi.org/10.1155/2013/576189 Text en Copyright © 2013 Yunying Cao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cao, Yunying Zhang, Qian Chen, Yanhong Zhao, Hua Lang, Youzhong Yu, Chunmei Yang, Jianchang Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis |
title | Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis |
title_full | Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis |
title_fullStr | Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis |
title_full_unstemmed | Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis |
title_short | Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis |
title_sort | identification of differential expression genes in leaves of rice (oryza sativa l.) in response to heat stress by cdna-aflp analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590577/ https://www.ncbi.nlm.nih.gov/pubmed/23509744 http://dx.doi.org/10.1155/2013/576189 |
work_keys_str_mv | AT caoyunying identificationofdifferentialexpressiongenesinleavesofriceoryzasativalinresponsetoheatstressbycdnaaflpanalysis AT zhangqian identificationofdifferentialexpressiongenesinleavesofriceoryzasativalinresponsetoheatstressbycdnaaflpanalysis AT chenyanhong identificationofdifferentialexpressiongenesinleavesofriceoryzasativalinresponsetoheatstressbycdnaaflpanalysis AT zhaohua identificationofdifferentialexpressiongenesinleavesofriceoryzasativalinresponsetoheatstressbycdnaaflpanalysis AT langyouzhong identificationofdifferentialexpressiongenesinleavesofriceoryzasativalinresponsetoheatstressbycdnaaflpanalysis AT yuchunmei identificationofdifferentialexpressiongenesinleavesofriceoryzasativalinresponsetoheatstressbycdnaaflpanalysis AT yangjianchang identificationofdifferentialexpressiongenesinleavesofriceoryzasativalinresponsetoheatstressbycdnaaflpanalysis |