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Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients

BACKGROUND: The B-cell translocation gene 2 (BTG2) is considered to act as a tumour-suppressor gene because of its antiproliferative and antimigratory activities. Higher levels of BTG2 expression in tumour cells have been linked to a better clinical outcome for several cancer entities. Here, we inve...

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Autores principales: Wagener, N, Bulkescher, J, Macher-Goeppinger, S, Karapanagiotou-Schenkel, I, Hatiboglu, G, Abdel-Rahim, M, Abol- Enein, H, Ghoneim, M A, Bastian, P J, Müller, S C, Haferkamp, A, Hohenfellner, M, Hoppe-Seyler, F, Hoppe-Seyler, K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590653/
https://www.ncbi.nlm.nih.gov/pubmed/23299537
http://dx.doi.org/10.1038/bjc.2012.573
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author Wagener, N
Bulkescher, J
Macher-Goeppinger, S
Karapanagiotou-Schenkel, I
Hatiboglu, G
Abdel-Rahim, M
Abol- Enein, H
Ghoneim, M A
Bastian, P J
Müller, S C
Haferkamp, A
Hohenfellner, M
Hoppe-Seyler, F
Hoppe-Seyler, K
author_facet Wagener, N
Bulkescher, J
Macher-Goeppinger, S
Karapanagiotou-Schenkel, I
Hatiboglu, G
Abdel-Rahim, M
Abol- Enein, H
Ghoneim, M A
Bastian, P J
Müller, S C
Haferkamp, A
Hohenfellner, M
Hoppe-Seyler, F
Hoppe-Seyler, K
author_sort Wagener, N
collection PubMed
description BACKGROUND: The B-cell translocation gene 2 (BTG2) is considered to act as a tumour-suppressor gene because of its antiproliferative and antimigratory activities. Higher levels of BTG2 expression in tumour cells have been linked to a better clinical outcome for several cancer entities. Here, we investigated the expression and function of BTG2 in bladder cancer. METHODS: The expression of BTG2 in bladder cancer cells was silenced by RNA interference. Cell motility was investigated by wound healing and Boyden chamber assays. The protein expression of BTG2 in bladder cancer was studied by immunohistochemistry. RESULTS: We observed that targeted suppression of BTG2 by RNA interference did not result in growth stimulation but led to a substantial inhibition of bladder cancer cell motility. Tissue microarray analyses of bladder cancer cystectomy specimens revealed that higher BTG2 expression levels within the tumours correlated strongly with a decreased cancer-specific survival for bladder cancer patients. CONCLUSION: These results indicate that endogenous BTG2 expression contributes to the migratory potential of bladder cancer cells. Moreover, high levels of BTG2 in bladder cancers are linked to decreased cancer-specific survival. These findings question the conception that BTG2 generally acts as a tumour suppressor and typically represents a favourable clinical marker for cancer patients.
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spelling pubmed-35906532014-03-05 Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients Wagener, N Bulkescher, J Macher-Goeppinger, S Karapanagiotou-Schenkel, I Hatiboglu, G Abdel-Rahim, M Abol- Enein, H Ghoneim, M A Bastian, P J Müller, S C Haferkamp, A Hohenfellner, M Hoppe-Seyler, F Hoppe-Seyler, K Br J Cancer Genetics and Genomics BACKGROUND: The B-cell translocation gene 2 (BTG2) is considered to act as a tumour-suppressor gene because of its antiproliferative and antimigratory activities. Higher levels of BTG2 expression in tumour cells have been linked to a better clinical outcome for several cancer entities. Here, we investigated the expression and function of BTG2 in bladder cancer. METHODS: The expression of BTG2 in bladder cancer cells was silenced by RNA interference. Cell motility was investigated by wound healing and Boyden chamber assays. The protein expression of BTG2 in bladder cancer was studied by immunohistochemistry. RESULTS: We observed that targeted suppression of BTG2 by RNA interference did not result in growth stimulation but led to a substantial inhibition of bladder cancer cell motility. Tissue microarray analyses of bladder cancer cystectomy specimens revealed that higher BTG2 expression levels within the tumours correlated strongly with a decreased cancer-specific survival for bladder cancer patients. CONCLUSION: These results indicate that endogenous BTG2 expression contributes to the migratory potential of bladder cancer cells. Moreover, high levels of BTG2 in bladder cancers are linked to decreased cancer-specific survival. These findings question the conception that BTG2 generally acts as a tumour suppressor and typically represents a favourable clinical marker for cancer patients. Nature Publishing Group 2013-03-05 2013-01-08 /pmc/articles/PMC3590653/ /pubmed/23299537 http://dx.doi.org/10.1038/bjc.2012.573 Text en Copyright © 2013 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Genetics and Genomics
Wagener, N
Bulkescher, J
Macher-Goeppinger, S
Karapanagiotou-Schenkel, I
Hatiboglu, G
Abdel-Rahim, M
Abol- Enein, H
Ghoneim, M A
Bastian, P J
Müller, S C
Haferkamp, A
Hohenfellner, M
Hoppe-Seyler, F
Hoppe-Seyler, K
Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients
title Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients
title_full Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients
title_fullStr Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients
title_full_unstemmed Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients
title_short Endogenous BTG2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients
title_sort endogenous btg2 expression stimulates migration of bladder cancer cells and correlates with poor clinical prognosis for bladder cancer patients
topic Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590653/
https://www.ncbi.nlm.nih.gov/pubmed/23299537
http://dx.doi.org/10.1038/bjc.2012.573
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