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Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes
BACKGROUND: In periodontology, Guided Tissue Regeneration (GTR) is based on the concept of providing a space for entry of cells with regenerative potential into the wound environment to initiate the regeneration of structures lost due to periodontal disease. First generation GTR membranes were prima...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590715/ https://www.ncbi.nlm.nih.gov/pubmed/23492817 http://dx.doi.org/10.4103/0972-124X.106883 |
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author | Thomas, Nebu G. Sanil, George P. Gopimohan, Rajmohan Prabhakaran, Jayachandran V. Thomas, George Panda, Amulya K. |
author_facet | Thomas, Nebu G. Sanil, George P. Gopimohan, Rajmohan Prabhakaran, Jayachandran V. Thomas, George Panda, Amulya K. |
author_sort | Thomas, Nebu G. |
collection | PubMed |
description | BACKGROUND: In periodontology, Guided Tissue Regeneration (GTR) is based on the concept of providing a space for entry of cells with regenerative potential into the wound environment to initiate the regeneration of structures lost due to periodontal disease. First generation GTR membranes were primarily non-absorbable membranes like expanded polytetrafluorethylene which required a second surgery for its removal. This led researchers to explore absorbable materials like collagen and synthetic biodegradable polymers to fabricate GTR membranes. In the present study, biodegradable Polylactic acid (PLA) is used to fabricate membranes with the potential to be used for GTR therapy. MATERIALS AND METHODS: Biocompatibility of the PLA membranes were evaluated in a subcutaneous guinea pig model. Antimicrobial effect of the drug-loaded PLA membranes were assessed against a drug-resistant Staphylococcus aureus bacterial isolate. The cytocompatibility of the drug-loaded membranes were evaluated using HeLa cell lines. RESULTS: The PLA membranes were shown to be biocompatible. The drug-loaded PLA membranes showed significant activity against the bacterial isolate. Among the drug-loaded membranes, tetracycline-loaded membrane showed minimal cellular toxicity. CONCLUSION: The results of this study indicate that biodegradable drug-releasing polylactide membranes have the potential to be used for periodontal regeneration. It has the necessary characteristics of a GTR membrane like biocompatibility, space maintaining ability, and tissue integration. Among the various antimicrobial agents loaded in the PLA membranes, tetracycline-loaded membranes exhibited minimal cellular toxicity against HeLa cells; at the same time showing significant activity against a pathogenic bacterium. |
format | Online Article Text |
id | pubmed-3590715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-35907152013-03-14 Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes Thomas, Nebu G. Sanil, George P. Gopimohan, Rajmohan Prabhakaran, Jayachandran V. Thomas, George Panda, Amulya K. J Indian Soc Periodontol Original Article BACKGROUND: In periodontology, Guided Tissue Regeneration (GTR) is based on the concept of providing a space for entry of cells with regenerative potential into the wound environment to initiate the regeneration of structures lost due to periodontal disease. First generation GTR membranes were primarily non-absorbable membranes like expanded polytetrafluorethylene which required a second surgery for its removal. This led researchers to explore absorbable materials like collagen and synthetic biodegradable polymers to fabricate GTR membranes. In the present study, biodegradable Polylactic acid (PLA) is used to fabricate membranes with the potential to be used for GTR therapy. MATERIALS AND METHODS: Biocompatibility of the PLA membranes were evaluated in a subcutaneous guinea pig model. Antimicrobial effect of the drug-loaded PLA membranes were assessed against a drug-resistant Staphylococcus aureus bacterial isolate. The cytocompatibility of the drug-loaded membranes were evaluated using HeLa cell lines. RESULTS: The PLA membranes were shown to be biocompatible. The drug-loaded PLA membranes showed significant activity against the bacterial isolate. Among the drug-loaded membranes, tetracycline-loaded membrane showed minimal cellular toxicity. CONCLUSION: The results of this study indicate that biodegradable drug-releasing polylactide membranes have the potential to be used for periodontal regeneration. It has the necessary characteristics of a GTR membrane like biocompatibility, space maintaining ability, and tissue integration. Among the various antimicrobial agents loaded in the PLA membranes, tetracycline-loaded membranes exhibited minimal cellular toxicity against HeLa cells; at the same time showing significant activity against a pathogenic bacterium. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3590715/ /pubmed/23492817 http://dx.doi.org/10.4103/0972-124X.106883 Text en Copyright: © Journal of Indian Society of Periodontology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Thomas, Nebu G. Sanil, George P. Gopimohan, Rajmohan Prabhakaran, Jayachandran V. Thomas, George Panda, Amulya K. Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes |
title | Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes |
title_full | Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes |
title_fullStr | Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes |
title_full_unstemmed | Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes |
title_short | Biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes |
title_sort | biocompatibility and cytotoxic evaluation of drug-loaded biodegradable guided tissue regeneration membranes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590715/ https://www.ncbi.nlm.nih.gov/pubmed/23492817 http://dx.doi.org/10.4103/0972-124X.106883 |
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