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Genome-Wide Analysis of DNA Methylation in Human Amnion
The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion fro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590748/ https://www.ncbi.nlm.nih.gov/pubmed/23533356 http://dx.doi.org/10.1155/2013/678156 |
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author | Kim, Jinsil Pitlick, Mitchell M. Christine, Paul J. Schaefer, Amanda R. Saleme, Cesar Comas, Belén Cosentino, Viviana Gadow, Enrique Murray, Jeffrey C. |
author_facet | Kim, Jinsil Pitlick, Mitchell M. Christine, Paul J. Schaefer, Amanda R. Saleme, Cesar Comas, Belén Cosentino, Viviana Gadow, Enrique Murray, Jeffrey C. |
author_sort | Kim, Jinsil |
collection | PubMed |
description | The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies. |
format | Online Article Text |
id | pubmed-3590748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35907482013-03-26 Genome-Wide Analysis of DNA Methylation in Human Amnion Kim, Jinsil Pitlick, Mitchell M. Christine, Paul J. Schaefer, Amanda R. Saleme, Cesar Comas, Belén Cosentino, Viviana Gadow, Enrique Murray, Jeffrey C. ScientificWorldJournal Research Article The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies. Hindawi Publishing Corporation 2013-02-19 /pmc/articles/PMC3590748/ /pubmed/23533356 http://dx.doi.org/10.1155/2013/678156 Text en Copyright © 2013 Jinsil Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kim, Jinsil Pitlick, Mitchell M. Christine, Paul J. Schaefer, Amanda R. Saleme, Cesar Comas, Belén Cosentino, Viviana Gadow, Enrique Murray, Jeffrey C. Genome-Wide Analysis of DNA Methylation in Human Amnion |
title | Genome-Wide Analysis of DNA Methylation in Human Amnion |
title_full | Genome-Wide Analysis of DNA Methylation in Human Amnion |
title_fullStr | Genome-Wide Analysis of DNA Methylation in Human Amnion |
title_full_unstemmed | Genome-Wide Analysis of DNA Methylation in Human Amnion |
title_short | Genome-Wide Analysis of DNA Methylation in Human Amnion |
title_sort | genome-wide analysis of dna methylation in human amnion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590748/ https://www.ncbi.nlm.nih.gov/pubmed/23533356 http://dx.doi.org/10.1155/2013/678156 |
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