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Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids
Hybridization and abiotic stress are natural agents hypothesized to influence activation and proliferation of transposable elements in wild populations. In this report, we examine the effects of these agents on expression dynamics of both quiescent and transcriptionally active sublineages of long te...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590766/ https://www.ncbi.nlm.nih.gov/pubmed/23335122 http://dx.doi.org/10.1093/gbe/evt006 |
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author | Ungerer, Mark C. Kawakami, Takeshi |
author_facet | Ungerer, Mark C. Kawakami, Takeshi |
author_sort | Ungerer, Mark C. |
collection | PubMed |
description | Hybridization and abiotic stress are natural agents hypothesized to influence activation and proliferation of transposable elements in wild populations. In this report, we examine the effects of these agents on expression dynamics of both quiescent and transcriptionally active sublineages of long terminal repeat (LTR) retrotransposons in wild sunflower species with a notable history of transposable element proliferation. For annual sunflower species Helianthus annuus and H. petiolaris, neither early generation hybridization nor abiotic stress, alone or in combination, induced transcriptional activation of quiescent sublineages of LTR retrotransposons. These treatments also failed to further induce expression of sublineages that are transcriptionally active; instead, expression of active sublineages in F1 and backcross hybrids was nondistinguishable from, or intermediate relative to, parental lines, and abiotic stress generally decreased normalized expression relative to controls. In contrast to findings for early generation hybridization between H. annuus and H. petiolaris, ancient sunflower hybrid species derived from these same two species and which have undergone massive proliferation events of LTR retrotransposons display 2× to 6× higher expression levels of transcriptionally active sublineages relative to parental sunflower species H. annuus and H. petiolaris. Implications and possible explanations for these findings are discussed. |
format | Online Article Text |
id | pubmed-3590766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-35907662013-03-07 Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids Ungerer, Mark C. Kawakami, Takeshi Genome Biol Evol Letter Hybridization and abiotic stress are natural agents hypothesized to influence activation and proliferation of transposable elements in wild populations. In this report, we examine the effects of these agents on expression dynamics of both quiescent and transcriptionally active sublineages of long terminal repeat (LTR) retrotransposons in wild sunflower species with a notable history of transposable element proliferation. For annual sunflower species Helianthus annuus and H. petiolaris, neither early generation hybridization nor abiotic stress, alone or in combination, induced transcriptional activation of quiescent sublineages of LTR retrotransposons. These treatments also failed to further induce expression of sublineages that are transcriptionally active; instead, expression of active sublineages in F1 and backcross hybrids was nondistinguishable from, or intermediate relative to, parental lines, and abiotic stress generally decreased normalized expression relative to controls. In contrast to findings for early generation hybridization between H. annuus and H. petiolaris, ancient sunflower hybrid species derived from these same two species and which have undergone massive proliferation events of LTR retrotransposons display 2× to 6× higher expression levels of transcriptionally active sublineages relative to parental sunflower species H. annuus and H. petiolaris. Implications and possible explanations for these findings are discussed. Oxford University Press 2013 2013-01-18 /pmc/articles/PMC3590766/ /pubmed/23335122 http://dx.doi.org/10.1093/gbe/evt006 Text en © The Author(s) 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Letter Ungerer, Mark C. Kawakami, Takeshi Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids |
title | Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids |
title_full | Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids |
title_fullStr | Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids |
title_full_unstemmed | Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids |
title_short | Transcriptional Dynamics of LTR Retrotransposons in Early Generation and Ancient Sunflower Hybrids |
title_sort | transcriptional dynamics of ltr retrotransposons in early generation and ancient sunflower hybrids |
topic | Letter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590766/ https://www.ncbi.nlm.nih.gov/pubmed/23335122 http://dx.doi.org/10.1093/gbe/evt006 |
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