Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen

Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic...

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Autores principales: Shahbazi-Gahrouei, Daryoush, Abdolahi, Mohammad, Zarkesh-Esfahani, Sayyed Hamid, Laurent, Sophie, Sermeus, Corine, Gruettner, Cordula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591120/
https://www.ncbi.nlm.nih.gov/pubmed/23484112
http://dx.doi.org/10.1155/2013/349408
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author Shahbazi-Gahrouei, Daryoush
Abdolahi, Mohammad
Zarkesh-Esfahani, Sayyed Hamid
Laurent, Sophie
Sermeus, Corine
Gruettner, Cordula
author_facet Shahbazi-Gahrouei, Daryoush
Abdolahi, Mohammad
Zarkesh-Esfahani, Sayyed Hamid
Laurent, Sophie
Sermeus, Corine
Gruettner, Cordula
author_sort Shahbazi-Gahrouei, Daryoush
collection PubMed
description Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis.
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spelling pubmed-35911202013-03-12 Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen Shahbazi-Gahrouei, Daryoush Abdolahi, Mohammad Zarkesh-Esfahani, Sayyed Hamid Laurent, Sophie Sermeus, Corine Gruettner, Cordula Biomed Res Int Research Article Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis. Hindawi Publishing Corporation 2013 2012-12-31 /pmc/articles/PMC3591120/ /pubmed/23484112 http://dx.doi.org/10.1155/2013/349408 Text en Copyright © 2013 Daryoush Shahbazi-Gahrouei et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shahbazi-Gahrouei, Daryoush
Abdolahi, Mohammad
Zarkesh-Esfahani, Sayyed Hamid
Laurent, Sophie
Sermeus, Corine
Gruettner, Cordula
Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen
title Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen
title_full Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen
title_fullStr Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen
title_full_unstemmed Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen
title_short Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen
title_sort functionalized magnetic nanoparticles for the detection and quantitative analysis of cell surface antigen
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591120/
https://www.ncbi.nlm.nih.gov/pubmed/23484112
http://dx.doi.org/10.1155/2013/349408
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