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Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria

Photodynamic antimicrobial chemotherapy based on photosensitizers activated by illumination is limited by poor penetration of visible light through skin and tissues. In order to overcome this problem, Rose Bengal was excited in the dark by 28 kHz ultrasound and was applied for inactivation of bacter...

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Autores principales: Nakonechny, Faina, Nisnevitch, Michael, Nitzan, Yeshayahu, Nisnevitch, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591171/
https://www.ncbi.nlm.nih.gov/pubmed/23509759
http://dx.doi.org/10.1155/2013/684930
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author Nakonechny, Faina
Nisnevitch, Michael
Nitzan, Yeshayahu
Nisnevitch, Marina
author_facet Nakonechny, Faina
Nisnevitch, Michael
Nitzan, Yeshayahu
Nisnevitch, Marina
author_sort Nakonechny, Faina
collection PubMed
description Photodynamic antimicrobial chemotherapy based on photosensitizers activated by illumination is limited by poor penetration of visible light through skin and tissues. In order to overcome this problem, Rose Bengal was excited in the dark by 28 kHz ultrasound and was applied for inactivation of bacteria. It is demonstrated, for the first time, that the sonodynamic technique is effective for eradication of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The net sonodynamic effect was calculated as a 3-4 log(10) reduction in bacteria concentration, depending on the cell and the Rose Bengal concentration and the treatment time. Sonodynamic treatment may become a novel and effective form of antimicrobial therapy and can be used for low-temperature sterilization of medical instruments and surgical accessories.
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spelling pubmed-35911712013-03-18 Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria Nakonechny, Faina Nisnevitch, Michael Nitzan, Yeshayahu Nisnevitch, Marina Biomed Res Int Research Article Photodynamic antimicrobial chemotherapy based on photosensitizers activated by illumination is limited by poor penetration of visible light through skin and tissues. In order to overcome this problem, Rose Bengal was excited in the dark by 28 kHz ultrasound and was applied for inactivation of bacteria. It is demonstrated, for the first time, that the sonodynamic technique is effective for eradication of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The net sonodynamic effect was calculated as a 3-4 log(10) reduction in bacteria concentration, depending on the cell and the Rose Bengal concentration and the treatment time. Sonodynamic treatment may become a novel and effective form of antimicrobial therapy and can be used for low-temperature sterilization of medical instruments and surgical accessories. Hindawi Publishing Corporation 2013 2012-12-19 /pmc/articles/PMC3591171/ /pubmed/23509759 http://dx.doi.org/10.1155/2013/684930 Text en Copyright © 2013 Faina Nakonechny et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nakonechny, Faina
Nisnevitch, Michael
Nitzan, Yeshayahu
Nisnevitch, Marina
Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria
title Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria
title_full Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria
title_fullStr Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria
title_full_unstemmed Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria
title_short Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria
title_sort sonodynamic excitation of rose bengal for eradication of gram-positive and gram-negative bacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591171/
https://www.ncbi.nlm.nih.gov/pubmed/23509759
http://dx.doi.org/10.1155/2013/684930
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