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Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria

Glutathione S-transferases (GSTs) play an important role in detoxification of xenobiotics in both prokaryotic and eukaryotic cells. In this study, four GSTs (LmGSTd1, LmGSTs5, LmGSTt1, and LmGSTu1) representing different classes were identified from the migratory locust, Locusta migratoria. These fo...

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Autores principales: Qin, Guohua, Jia, Miao, Liu, Ting, Zhang, Xueyao, Guo, Yaping, Zhu, Kun Yan, Ma, Enbo, Zhang, Jianzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591310/
https://www.ncbi.nlm.nih.gov/pubmed/23505503
http://dx.doi.org/10.1371/journal.pone.0058410
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author Qin, Guohua
Jia, Miao
Liu, Ting
Zhang, Xueyao
Guo, Yaping
Zhu, Kun Yan
Ma, Enbo
Zhang, Jianzhen
author_facet Qin, Guohua
Jia, Miao
Liu, Ting
Zhang, Xueyao
Guo, Yaping
Zhu, Kun Yan
Ma, Enbo
Zhang, Jianzhen
author_sort Qin, Guohua
collection PubMed
description Glutathione S-transferases (GSTs) play an important role in detoxification of xenobiotics in both prokaryotic and eukaryotic cells. In this study, four GSTs (LmGSTd1, LmGSTs5, LmGSTt1, and LmGSTu1) representing different classes were identified from the migratory locust, Locusta migratoria. These four proteins were heterologously expressed in Escherichia coli as soluble fusion proteins, purified by Ni(2+)-nitrilotriacetic acid agarose column and biochemically characterized. LmGSTd1, LmGSTs5, and LmGSTu1 showed high activities with 1-chloro-2, 4-dinitrobenzene (CDNB), detectable activity with p-nitro-benzyl chloride (p-NBC) and 1, 2-dichloro-4-nitrobenzene (DCNB), whereas LmGSTt1 showed high activity with p-NBC and detectable activity with CDNB. The optimal pH of the locust GSTs ranged between 7.0 to 9.0. Ethacrynic acid and reactive blue effectively inhibited all four GSTs. LmGSTs5 was most sensitive to heavy metals (Cu(2+) and Cd(2+)). The maximum expression of the four GSTs was observed in Malpighian tubules and fat bodies as evaluated by western blot. The nymph mortalities after carbaryl treatment increased by 28 and 12% after LmGSTs5 and LmGSTu1 were silenced, respectively. The nymph mortalities after malathion and chlorpyrifos treatments increased by 26 and 18% after LmGSTs5 and LmGSTu1 were silenced, respectively. These results suggest that sigma GSTs in L. migratoria play a significant role in carbaryl detoxification, whereas some of other GSTs may also involve in the detoxification of carbaryl and chlorpyrifos.
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spelling pubmed-35913102013-03-15 Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria Qin, Guohua Jia, Miao Liu, Ting Zhang, Xueyao Guo, Yaping Zhu, Kun Yan Ma, Enbo Zhang, Jianzhen PLoS One Research Article Glutathione S-transferases (GSTs) play an important role in detoxification of xenobiotics in both prokaryotic and eukaryotic cells. In this study, four GSTs (LmGSTd1, LmGSTs5, LmGSTt1, and LmGSTu1) representing different classes were identified from the migratory locust, Locusta migratoria. These four proteins were heterologously expressed in Escherichia coli as soluble fusion proteins, purified by Ni(2+)-nitrilotriacetic acid agarose column and biochemically characterized. LmGSTd1, LmGSTs5, and LmGSTu1 showed high activities with 1-chloro-2, 4-dinitrobenzene (CDNB), detectable activity with p-nitro-benzyl chloride (p-NBC) and 1, 2-dichloro-4-nitrobenzene (DCNB), whereas LmGSTt1 showed high activity with p-NBC and detectable activity with CDNB. The optimal pH of the locust GSTs ranged between 7.0 to 9.0. Ethacrynic acid and reactive blue effectively inhibited all four GSTs. LmGSTs5 was most sensitive to heavy metals (Cu(2+) and Cd(2+)). The maximum expression of the four GSTs was observed in Malpighian tubules and fat bodies as evaluated by western blot. The nymph mortalities after carbaryl treatment increased by 28 and 12% after LmGSTs5 and LmGSTu1 were silenced, respectively. The nymph mortalities after malathion and chlorpyrifos treatments increased by 26 and 18% after LmGSTs5 and LmGSTu1 were silenced, respectively. These results suggest that sigma GSTs in L. migratoria play a significant role in carbaryl detoxification, whereas some of other GSTs may also involve in the detoxification of carbaryl and chlorpyrifos. Public Library of Science 2013-03-07 /pmc/articles/PMC3591310/ /pubmed/23505503 http://dx.doi.org/10.1371/journal.pone.0058410 Text en © 2013 Qin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Qin, Guohua
Jia, Miao
Liu, Ting
Zhang, Xueyao
Guo, Yaping
Zhu, Kun Yan
Ma, Enbo
Zhang, Jianzhen
Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria
title Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria
title_full Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria
title_fullStr Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria
title_full_unstemmed Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria
title_short Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria
title_sort characterization and functional analysis of four glutathione s-transferases from the migratory locust, locusta migratoria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591310/
https://www.ncbi.nlm.nih.gov/pubmed/23505503
http://dx.doi.org/10.1371/journal.pone.0058410
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