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Differentiating Intracellular from Extracellular Alkaline Phosphatase Activity in Soil by Sonication

Differentiating intracellular from extracellular enzyme activity is important in soil enzymology, but not easy. Here, we report on an adjusted sonication method for the separation of intracellular from extracellular phosphatase activity in soil. Under optimal sonication conditions [soil:water ratio...

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Detalles Bibliográficos
Autores principales: Qin, Shuping, Hu, Chunsheng, Oenema, Oene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591387/
https://www.ncbi.nlm.nih.gov/pubmed/23527006
http://dx.doi.org/10.1371/journal.pone.0058691