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Differentiating Intracellular from Extracellular Alkaline Phosphatase Activity in Soil by Sonication
Differentiating intracellular from extracellular enzyme activity is important in soil enzymology, but not easy. Here, we report on an adjusted sonication method for the separation of intracellular from extracellular phosphatase activity in soil. Under optimal sonication conditions [soil:water ratio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591387/ https://www.ncbi.nlm.nih.gov/pubmed/23527006 http://dx.doi.org/10.1371/journal.pone.0058691 |