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Identification of Intact High Molecular Weight Glutenin Subunits from the Wheat Proteome Using Combined Liquid Chromatography-Electrospray Ionization Mass Spectrometry
The present paper describes a method for the identification of intact high molecular weight glutenin subunits (HMW-GS), the quality determining proteins from the wheat storage proteome. The method includes isolation of HMW-GS from wheat flour, further separation of HMW-GS by reversed-phase high-perf...
Autores principales: | Lagrain, Bert, Brunnbauer, Markus, Rombouts, Ine, Koehler, Peter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592795/ https://www.ncbi.nlm.nih.gov/pubmed/23520527 http://dx.doi.org/10.1371/journal.pone.0058682 |
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