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Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract

Oxidative stress and inflammation play important roles in disease development. This study intended to evaluate the anti-inflammatory and antioxidant potential of Echium plantagineum L. bee pollen to support its claimed health beneficial effects. The hydromethanol extract efficiently scavenged nitric...

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Autores principales: Moita, Eduarda, Gil-Izquierdo, Angel, Sousa, Carla, Ferreres, Federico, Silva, Luís R., Valentão, Patrícia, Domínguez-Perles, Raúl, Baenas, Nieves, Andrade, Paula B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592834/
https://www.ncbi.nlm.nih.gov/pubmed/23520554
http://dx.doi.org/10.1371/journal.pone.0059131
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author Moita, Eduarda
Gil-Izquierdo, Angel
Sousa, Carla
Ferreres, Federico
Silva, Luís R.
Valentão, Patrícia
Domínguez-Perles, Raúl
Baenas, Nieves
Andrade, Paula B.
author_facet Moita, Eduarda
Gil-Izquierdo, Angel
Sousa, Carla
Ferreres, Federico
Silva, Luís R.
Valentão, Patrícia
Domínguez-Perles, Raúl
Baenas, Nieves
Andrade, Paula B.
author_sort Moita, Eduarda
collection PubMed
description Oxidative stress and inflammation play important roles in disease development. This study intended to evaluate the anti-inflammatory and antioxidant potential of Echium plantagineum L. bee pollen to support its claimed health beneficial effects. The hydromethanol extract efficiently scavenged nitric oxide ((•)NO) although against superoxide (O(2) (•−)) it behaved as antioxidant at lower concentrations and as pro-oxidant at higher concentrations. The anti-inflammatory potential was evaluated in LPS-stimulated macrophages. The levels of (•)NO and L-citrulline decreased for all extract concentrations tested, while the levels of prostaglandins, their metabolites and isoprostanes, evaluated by UPLC-MS, decreased with low extract concentrations. So, E. plantagineum bee pollen extract can exert anti-inflammatory activity by reducing (•)NO and prostaglandins. The extract is able to scavenge the reactive species (•)NO and O(2) (•−) and reduce markers of oxidative stress in cells at low concentrations.
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spelling pubmed-35928342013-03-21 Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract Moita, Eduarda Gil-Izquierdo, Angel Sousa, Carla Ferreres, Federico Silva, Luís R. Valentão, Patrícia Domínguez-Perles, Raúl Baenas, Nieves Andrade, Paula B. PLoS One Research Article Oxidative stress and inflammation play important roles in disease development. This study intended to evaluate the anti-inflammatory and antioxidant potential of Echium plantagineum L. bee pollen to support its claimed health beneficial effects. The hydromethanol extract efficiently scavenged nitric oxide ((•)NO) although against superoxide (O(2) (•−)) it behaved as antioxidant at lower concentrations and as pro-oxidant at higher concentrations. The anti-inflammatory potential was evaluated in LPS-stimulated macrophages. The levels of (•)NO and L-citrulline decreased for all extract concentrations tested, while the levels of prostaglandins, their metabolites and isoprostanes, evaluated by UPLC-MS, decreased with low extract concentrations. So, E. plantagineum bee pollen extract can exert anti-inflammatory activity by reducing (•)NO and prostaglandins. The extract is able to scavenge the reactive species (•)NO and O(2) (•−) and reduce markers of oxidative stress in cells at low concentrations. Public Library of Science 2013-03-08 /pmc/articles/PMC3592834/ /pubmed/23520554 http://dx.doi.org/10.1371/journal.pone.0059131 Text en © 2013 Moita et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Moita, Eduarda
Gil-Izquierdo, Angel
Sousa, Carla
Ferreres, Federico
Silva, Luís R.
Valentão, Patrícia
Domínguez-Perles, Raúl
Baenas, Nieves
Andrade, Paula B.
Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract
title Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract
title_full Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract
title_fullStr Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract
title_full_unstemmed Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract
title_short Integrated Analysis of COX-2 and iNOS Derived Inflammatory Mediators in LPS-Stimulated RAW Macrophages Pre-Exposed to Echium plantagineum L. Bee Pollen Extract
title_sort integrated analysis of cox-2 and inos derived inflammatory mediators in lps-stimulated raw macrophages pre-exposed to echium plantagineum l. bee pollen extract
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592834/
https://www.ncbi.nlm.nih.gov/pubmed/23520554
http://dx.doi.org/10.1371/journal.pone.0059131
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