Cargando…
Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue
Apurinic/apyrimidinic endonuclease-1 (APE-1), a key enzyme responsible for DNA base excision repair (BER), has been linked to cancer chemoradiosensitivity. The phosphorylation of p65 plays a role in the activation of this pathway. In this study, we investigated APE-1 expression and its interaction w...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Society for Free Radical Research Japan
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593127/ https://www.ncbi.nlm.nih.gov/pubmed/23525727 http://dx.doi.org/10.3164/jcbn.12-95 |
_version_ | 1782262222859272192 |
---|---|
author | Song, Junmin Futagami, Seiji Nagoya, Hiroyuki Kawagoe, Tetsuro Yamawaki, Hiroshi Kodaka, Yasuhiro Tatsuguchi, Atsushi Gudis, Katya Wakabayashi, Taiga Yonezawa, Masaoki Shimpuku, Mayumi Watarai, Yasuhiko Iwakiri, Katsuhiko Hoshihara, Yoshio Makino, Hiroshi Miyashita, Masao Tsuchiya, Shinichi Li, Yan E. Crowe, Sheila Sakamoto, Choitsu |
author_facet | Song, Junmin Futagami, Seiji Nagoya, Hiroyuki Kawagoe, Tetsuro Yamawaki, Hiroshi Kodaka, Yasuhiro Tatsuguchi, Atsushi Gudis, Katya Wakabayashi, Taiga Yonezawa, Masaoki Shimpuku, Mayumi Watarai, Yasuhiko Iwakiri, Katsuhiko Hoshihara, Yoshio Makino, Hiroshi Miyashita, Masao Tsuchiya, Shinichi Li, Yan E. Crowe, Sheila Sakamoto, Choitsu |
author_sort | Song, Junmin |
collection | PubMed |
description | Apurinic/apyrimidinic endonuclease-1 (APE-1), a key enzyme responsible for DNA base excision repair (BER), has been linked to cancer chemoradiosensitivity. The phosphorylation of p65 plays a role in the activation of this pathway. In this study, we investigated APE-1 expression and its interaction with p65 in esophageal squamous cell carcinoma (ESCC) tissue. The expression of APE-1, p65, p65 nuclear localization sequence (p65-NLS), and monocyte chemoattractant protein-1 (MCP-1) was assessed by immunohistochemical analysis in 67 human ESCC tissue samples. Real-time PCR and western blotting were also performed. p65 siRNA was evaluated to determine the role of p65 in the regulation of APE-1 expression. We found nuclear localization of APE-1 in 89.6% (60/67) of ESCC tissue samples. We also observed the colocalization of p65-NLS and APE-1 in esophageal cancer tissue. In KYSE220 cells, pretreatment of MG-132 significantly abrogated upregulation of p65 and APE-1 levels induced by MCP-1, and treatment with 10 and 20 nM p65 siRNA significantly inhibited APE-1 mRNA expression. siRNA for p65 treatment significantly increased the apoptotic index in 5-FU-treated KYSE220 cells. We conclude that APE-1 is overexpressed and mainly localized in the nuclear compartment of cancer cells, and partly regulated by p65 in the NF-κB pathway in ESCC tissue. |
format | Online Article Text |
id | pubmed-3593127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-35931272013-03-22 Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue Song, Junmin Futagami, Seiji Nagoya, Hiroyuki Kawagoe, Tetsuro Yamawaki, Hiroshi Kodaka, Yasuhiro Tatsuguchi, Atsushi Gudis, Katya Wakabayashi, Taiga Yonezawa, Masaoki Shimpuku, Mayumi Watarai, Yasuhiko Iwakiri, Katsuhiko Hoshihara, Yoshio Makino, Hiroshi Miyashita, Masao Tsuchiya, Shinichi Li, Yan E. Crowe, Sheila Sakamoto, Choitsu J Clin Biochem Nutr Original Article Apurinic/apyrimidinic endonuclease-1 (APE-1), a key enzyme responsible for DNA base excision repair (BER), has been linked to cancer chemoradiosensitivity. The phosphorylation of p65 plays a role in the activation of this pathway. In this study, we investigated APE-1 expression and its interaction with p65 in esophageal squamous cell carcinoma (ESCC) tissue. The expression of APE-1, p65, p65 nuclear localization sequence (p65-NLS), and monocyte chemoattractant protein-1 (MCP-1) was assessed by immunohistochemical analysis in 67 human ESCC tissue samples. Real-time PCR and western blotting were also performed. p65 siRNA was evaluated to determine the role of p65 in the regulation of APE-1 expression. We found nuclear localization of APE-1 in 89.6% (60/67) of ESCC tissue samples. We also observed the colocalization of p65-NLS and APE-1 in esophageal cancer tissue. In KYSE220 cells, pretreatment of MG-132 significantly abrogated upregulation of p65 and APE-1 levels induced by MCP-1, and treatment with 10 and 20 nM p65 siRNA significantly inhibited APE-1 mRNA expression. siRNA for p65 treatment significantly increased the apoptotic index in 5-FU-treated KYSE220 cells. We conclude that APE-1 is overexpressed and mainly localized in the nuclear compartment of cancer cells, and partly regulated by p65 in the NF-κB pathway in ESCC tissue. the Society for Free Radical Research Japan 2013-03 2013-03-01 /pmc/articles/PMC3593127/ /pubmed/23525727 http://dx.doi.org/10.3164/jcbn.12-95 Text en Copyright © 2013 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Song, Junmin Futagami, Seiji Nagoya, Hiroyuki Kawagoe, Tetsuro Yamawaki, Hiroshi Kodaka, Yasuhiro Tatsuguchi, Atsushi Gudis, Katya Wakabayashi, Taiga Yonezawa, Masaoki Shimpuku, Mayumi Watarai, Yasuhiko Iwakiri, Katsuhiko Hoshihara, Yoshio Makino, Hiroshi Miyashita, Masao Tsuchiya, Shinichi Li, Yan E. Crowe, Sheila Sakamoto, Choitsu Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue |
title | Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue |
title_full | Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue |
title_fullStr | Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue |
title_full_unstemmed | Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue |
title_short | Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue |
title_sort | apurinic/apyrimidinic endonuclease-1 (ape-1) is overexpressed via the activation of nf-κb-p65 in mcp-1-positive esophageal squamous cell carcinoma tissue |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593127/ https://www.ncbi.nlm.nih.gov/pubmed/23525727 http://dx.doi.org/10.3164/jcbn.12-95 |
work_keys_str_mv | AT songjunmin apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT futagamiseiji apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT nagoyahiroyuki apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT kawagoetetsuro apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT yamawakihiroshi apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT kodakayasuhiro apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT tatsuguchiatsushi apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT gudiskatya apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT wakabayashitaiga apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT yonezawamasaoki apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT shimpukumayumi apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT wataraiyasuhiko apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT iwakirikatsuhiko apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT hoshiharayoshio apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT makinohiroshi apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT miyashitamasao apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT tsuchiyashinichi apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT liyan apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT ecrowesheila apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue AT sakamotochoitsu apurinicapyrimidinicendonuclease1ape1isoverexpressedviatheactivationofnfkbp65inmcp1positiveesophagealsquamouscellcarcinomatissue |