Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor

OBJECTIVE: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic...

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Autores principales: Rassouli, Hassan, Tabe Bordbar, Mohammad Sharif, Rezaei Larijani, Mehran, Pakzad, Mohammad, Baharvand, Hossein, Hosseini Salekdeh, Ghasem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593933/
https://www.ncbi.nlm.nih.gov/pubmed/23577308
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author Rassouli, Hassan
Tabe Bordbar, Mohammad Sharif
Rezaei Larijani, Mehran
Pakzad, Mohammad
Baharvand, Hossein
Hosseini Salekdeh, Ghasem
author_facet Rassouli, Hassan
Tabe Bordbar, Mohammad Sharif
Rezaei Larijani, Mehran
Pakzad, Mohammad
Baharvand, Hossein
Hosseini Salekdeh, Ghasem
author_sort Rassouli, Hassan
collection PubMed
description OBJECTIVE: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). MATERIALS AND METHODS: In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability. RESULTS: This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo. CONCLUSION: The results show no significant difference in function between our in-house produced and commercialized bFGF.
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spelling pubmed-35939332013-04-10 Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor Rassouli, Hassan Tabe Bordbar, Mohammad Sharif Rezaei Larijani, Mehran Pakzad, Mohammad Baharvand, Hossein Hosseini Salekdeh, Ghasem Cell J Research Article OBJECTIVE: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). MATERIALS AND METHODS: In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability. RESULTS: This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo. CONCLUSION: The results show no significant difference in function between our in-house produced and commercialized bFGF. Royan Institute 2013 2013-02-20 /pmc/articles/PMC3593933/ /pubmed/23577308 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rassouli, Hassan
Tabe Bordbar, Mohammad Sharif
Rezaei Larijani, Mehran
Pakzad, Mohammad
Baharvand, Hossein
Hosseini Salekdeh, Ghasem
Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor
title Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor
title_full Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor
title_fullStr Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor
title_full_unstemmed Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor
title_short Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor
title_sort cloning, expression and functional characterization of in-house prepared human basic fibroblast growth factor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593933/
https://www.ncbi.nlm.nih.gov/pubmed/23577308
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