Characterization and Quantification of Intact 26S Proteasome Proteins by Real-Time Measurement of Intrinsic Fluorescence Prior to Top-down Mass Spectrometry

Quantification of gas-phase intact protein ions by mass spectrometry (MS) is impeded by highly-variable ionization, ion transmission, and ion detection efficiencies. Therefore, quantification of proteins using MS-associated techniques is almost exclusively done after proteolysis where peptides serve...

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Detalles Bibliográficos
Autores principales: Russell, Jason D., Scalf, Mark, Book, Adam J., Ladror, Daniel T., Vierstra, Richard D., Smith, Lloyd M., Coon, Joshua J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594244/
https://www.ncbi.nlm.nih.gov/pubmed/23536786
http://dx.doi.org/10.1371/journal.pone.0058157

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