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Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria

β-Barrel proteins are present only in the outer membranes of Gram-negative bacteria, chloroplasts and mitochondria. Fungal mitochondria were shown to readily import and assemble bacterial β-barrel proteins, but human mitochondria exhibit certain selectivity. Whereas enterobacterial β-barrel proteins...

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Autores principales: Ott, Christine, Utech, Mandy, Goetz, Monika, Rudel, Thomas, Kozjak-Pavlovic, Vera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596097/
https://www.ncbi.nlm.nih.gov/pubmed/23368846
http://dx.doi.org/10.1042/BSR20130007
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author Ott, Christine
Utech, Mandy
Goetz, Monika
Rudel, Thomas
Kozjak-Pavlovic, Vera
author_facet Ott, Christine
Utech, Mandy
Goetz, Monika
Rudel, Thomas
Kozjak-Pavlovic, Vera
author_sort Ott, Christine
collection PubMed
description β-Barrel proteins are present only in the outer membranes of Gram-negative bacteria, chloroplasts and mitochondria. Fungal mitochondria were shown to readily import and assemble bacterial β-barrel proteins, but human mitochondria exhibit certain selectivity. Whereas enterobacterial β-barrel proteins are not imported, neisserial ones are. Of those, solely neisserial Omp85 is integrated into the outer membrane of mitochondria. In this study, we wanted to identify the signal that targets neisserial β-barrel proteins to mitochondria. We exchanged parts of neisserial Omp85 and PorB with their Escherichia coli homologues BamA and OmpC. For PorB, we could show that its C-terminal quarter can direct OmpC to mitochondria. In the case of Omp85, we could identify several amino acids of the C-terminal β-sorting signal as crucial for mitochondrial targeting. Additionally, we found that at least two POTRA (polypeptide-transport associated) domains and not only the β-sorting signal of Omp85 are needed for its membrane integration and function in human mitochondria. We conclude that the signal that directs neisserial β-barrel proteins to mitochondria is not conserved between these proteins. Furthermore, a linear mitochondrial targeting signal probably does not exist. It is possible that the secondary structure of β-barrel proteins plays a role in directing these proteins to mitochondria.
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spelling pubmed-35960972013-03-18 Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria Ott, Christine Utech, Mandy Goetz, Monika Rudel, Thomas Kozjak-Pavlovic, Vera Biosci Rep Original Paper β-Barrel proteins are present only in the outer membranes of Gram-negative bacteria, chloroplasts and mitochondria. Fungal mitochondria were shown to readily import and assemble bacterial β-barrel proteins, but human mitochondria exhibit certain selectivity. Whereas enterobacterial β-barrel proteins are not imported, neisserial ones are. Of those, solely neisserial Omp85 is integrated into the outer membrane of mitochondria. In this study, we wanted to identify the signal that targets neisserial β-barrel proteins to mitochondria. We exchanged parts of neisserial Omp85 and PorB with their Escherichia coli homologues BamA and OmpC. For PorB, we could show that its C-terminal quarter can direct OmpC to mitochondria. In the case of Omp85, we could identify several amino acids of the C-terminal β-sorting signal as crucial for mitochondrial targeting. Additionally, we found that at least two POTRA (polypeptide-transport associated) domains and not only the β-sorting signal of Omp85 are needed for its membrane integration and function in human mitochondria. We conclude that the signal that directs neisserial β-barrel proteins to mitochondria is not conserved between these proteins. Furthermore, a linear mitochondrial targeting signal probably does not exist. It is possible that the secondary structure of β-barrel proteins plays a role in directing these proteins to mitochondria. Portland Press Ltd. 2013-03-13 /pmc/articles/PMC3596097/ /pubmed/23368846 http://dx.doi.org/10.1042/BSR20130007 Text en © 2013 The Author(s). http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Ott, Christine
Utech, Mandy
Goetz, Monika
Rudel, Thomas
Kozjak-Pavlovic, Vera
Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria
title Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria
title_full Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria
title_fullStr Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria
title_full_unstemmed Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria
title_short Requirements for the import of neisserial Omp85 into the outer membrane of human mitochondria
title_sort requirements for the import of neisserial omp85 into the outer membrane of human mitochondria
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596097/
https://www.ncbi.nlm.nih.gov/pubmed/23368846
http://dx.doi.org/10.1042/BSR20130007
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