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p120-catenin and β-catenin differentially regulate cadherin adhesive function

Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, the specific functional contributions of the catenins to the establishment of strong adhesion are not fully underst...

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Autores principales: Oas, Rebecca G., Nanes, Benjamin A., Esimai, Chimdimnma C., Vincent, Peter A., García, Andrés J., Kowalczyk, Andrew P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596243/
https://www.ncbi.nlm.nih.gov/pubmed/23325790
http://dx.doi.org/10.1091/mbc.E12-06-0471
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author Oas, Rebecca G.
Nanes, Benjamin A.
Esimai, Chimdimnma C.
Vincent, Peter A.
García, Andrés J.
Kowalczyk, Andrew P.
author_facet Oas, Rebecca G.
Nanes, Benjamin A.
Esimai, Chimdimnma C.
Vincent, Peter A.
García, Andrés J.
Kowalczyk, Andrew P.
author_sort Oas, Rebecca G.
collection PubMed
description Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, the specific functional contributions of the catenins to the establishment of strong adhesion are not fully understood. Here we use bioengineering approaches to identify the roles of cadherin–catenin interactions in promoting strong cellular adhesion and the ability of the cells to spread on an adhesive surface. Our results demonstrate that the domain of VE-cadherin that binds to β-catenin is required for the establishment of strong steady-state adhesion strength. Surprisingly, p120 binding to the cadherin tail had no effect on the strength of adhesion when the available adhesive area was limited. Instead, the binding of VE-cadherin to p120 regulates adhesive contact area in a Rac1-dependent manner. These findings reveal that p120 and β-catenin have distinct but complementary roles in strengthening cadherin-mediated adhesion.
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spelling pubmed-35962432013-05-30 p120-catenin and β-catenin differentially regulate cadherin adhesive function Oas, Rebecca G. Nanes, Benjamin A. Esimai, Chimdimnma C. Vincent, Peter A. García, Andrés J. Kowalczyk, Andrew P. Mol Biol Cell Articles Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, the specific functional contributions of the catenins to the establishment of strong adhesion are not fully understood. Here we use bioengineering approaches to identify the roles of cadherin–catenin interactions in promoting strong cellular adhesion and the ability of the cells to spread on an adhesive surface. Our results demonstrate that the domain of VE-cadherin that binds to β-catenin is required for the establishment of strong steady-state adhesion strength. Surprisingly, p120 binding to the cadherin tail had no effect on the strength of adhesion when the available adhesive area was limited. Instead, the binding of VE-cadherin to p120 regulates adhesive contact area in a Rac1-dependent manner. These findings reveal that p120 and β-catenin have distinct but complementary roles in strengthening cadherin-mediated adhesion. The American Society for Cell Biology 2013-03-15 /pmc/articles/PMC3596243/ /pubmed/23325790 http://dx.doi.org/10.1091/mbc.E12-06-0471 Text en © 2013 Oas et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Oas, Rebecca G.
Nanes, Benjamin A.
Esimai, Chimdimnma C.
Vincent, Peter A.
García, Andrés J.
Kowalczyk, Andrew P.
p120-catenin and β-catenin differentially regulate cadherin adhesive function
title p120-catenin and β-catenin differentially regulate cadherin adhesive function
title_full p120-catenin and β-catenin differentially regulate cadherin adhesive function
title_fullStr p120-catenin and β-catenin differentially regulate cadherin adhesive function
title_full_unstemmed p120-catenin and β-catenin differentially regulate cadherin adhesive function
title_short p120-catenin and β-catenin differentially regulate cadherin adhesive function
title_sort p120-catenin and β-catenin differentially regulate cadherin adhesive function
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596243/
https://www.ncbi.nlm.nih.gov/pubmed/23325790
http://dx.doi.org/10.1091/mbc.E12-06-0471
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