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p120-catenin and β-catenin differentially regulate cadherin adhesive function
Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, the specific functional contributions of the catenins to the establishment of strong adhesion are not fully underst...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596243/ https://www.ncbi.nlm.nih.gov/pubmed/23325790 http://dx.doi.org/10.1091/mbc.E12-06-0471 |
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author | Oas, Rebecca G. Nanes, Benjamin A. Esimai, Chimdimnma C. Vincent, Peter A. García, Andrés J. Kowalczyk, Andrew P. |
author_facet | Oas, Rebecca G. Nanes, Benjamin A. Esimai, Chimdimnma C. Vincent, Peter A. García, Andrés J. Kowalczyk, Andrew P. |
author_sort | Oas, Rebecca G. |
collection | PubMed |
description | Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, the specific functional contributions of the catenins to the establishment of strong adhesion are not fully understood. Here we use bioengineering approaches to identify the roles of cadherin–catenin interactions in promoting strong cellular adhesion and the ability of the cells to spread on an adhesive surface. Our results demonstrate that the domain of VE-cadherin that binds to β-catenin is required for the establishment of strong steady-state adhesion strength. Surprisingly, p120 binding to the cadherin tail had no effect on the strength of adhesion when the available adhesive area was limited. Instead, the binding of VE-cadherin to p120 regulates adhesive contact area in a Rac1-dependent manner. These findings reveal that p120 and β-catenin have distinct but complementary roles in strengthening cadherin-mediated adhesion. |
format | Online Article Text |
id | pubmed-3596243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-35962432013-05-30 p120-catenin and β-catenin differentially regulate cadherin adhesive function Oas, Rebecca G. Nanes, Benjamin A. Esimai, Chimdimnma C. Vincent, Peter A. García, Andrés J. Kowalczyk, Andrew P. Mol Biol Cell Articles Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, the specific functional contributions of the catenins to the establishment of strong adhesion are not fully understood. Here we use bioengineering approaches to identify the roles of cadherin–catenin interactions in promoting strong cellular adhesion and the ability of the cells to spread on an adhesive surface. Our results demonstrate that the domain of VE-cadherin that binds to β-catenin is required for the establishment of strong steady-state adhesion strength. Surprisingly, p120 binding to the cadherin tail had no effect on the strength of adhesion when the available adhesive area was limited. Instead, the binding of VE-cadherin to p120 regulates adhesive contact area in a Rac1-dependent manner. These findings reveal that p120 and β-catenin have distinct but complementary roles in strengthening cadherin-mediated adhesion. The American Society for Cell Biology 2013-03-15 /pmc/articles/PMC3596243/ /pubmed/23325790 http://dx.doi.org/10.1091/mbc.E12-06-0471 Text en © 2013 Oas et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Oas, Rebecca G. Nanes, Benjamin A. Esimai, Chimdimnma C. Vincent, Peter A. García, Andrés J. Kowalczyk, Andrew P. p120-catenin and β-catenin differentially regulate cadherin adhesive function |
title | p120-catenin and β-catenin differentially regulate cadherin adhesive function |
title_full | p120-catenin and β-catenin differentially regulate cadherin adhesive function |
title_fullStr | p120-catenin and β-catenin differentially regulate cadherin adhesive function |
title_full_unstemmed | p120-catenin and β-catenin differentially regulate cadherin adhesive function |
title_short | p120-catenin and β-catenin differentially regulate cadherin adhesive function |
title_sort | p120-catenin and β-catenin differentially regulate cadherin adhesive function |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596243/ https://www.ncbi.nlm.nih.gov/pubmed/23325790 http://dx.doi.org/10.1091/mbc.E12-06-0471 |
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